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生物传感和诊断中的10-23DNA酶:应用,挑战和未来方向

Connor Nurmi^1,2, Jake Brill³, Sanne Roumans¹

¹Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada.

Angewandte Chemie (International ed. in English)

|February 28, 2026

概括

10-23DNA酶是生物感知的一个强大工具,但它的性能受到环境因素的限制. 本综述探讨了克服这些挑战的策略,以改善诊断应用.

科学领域:

生物化学生物化学
分子生物学分子生物学
纳米技术纳米技术

背景情况:

DNA酶,特别是10-23 DNA酶,提供可编程和具有成本效益的生物感知能力.
10-23DNAzyme是一种广泛使用的RNA分裂酶,具有高的催化效率和适应性.
诸如次优条件和核酶干扰等局限性阻碍了10-23DNA酶在临床诊断中的性能.

研究的目的:

审查10-23DNA酶在各种生物传感平台中的集成.
突出激活策略和10-23DNA酶在分子识别和信号报告中的双重作用.
讨论挑战和新出现的解决方案,以推进10-23DNA酶的应用.

主要方法:

审查使用10-23DNA酶的当代生物传感和诊断系统.
对10-23DNA酶的直接和受调激活策略的分析.
解决方案的检查,包括化学修改,纳米粒子保护和先进的传感器架构.

主要成果:

10-23 DNA酶集成到色度,光,电化学,电化学发光和细胞内传感器中.
使用直接和受调节的激活策略,利用10-23DNA酶的双重作用.
新兴的解决方案解决了催化性能,稳定性和测试工作流程方面的挑战.

结论:

10-23 DNA酶显示出下一代生物传感和诊断的巨大潜力.
通过创新策略克服局限性对于在 point-of-care 格式中广泛采用至关重要.
化学修饰,纳米技术和传感器设计的进一步进步将提高10-23DNA酶的实用性.

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