相关实验视频

Updated: Mar 1, 2026

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Published on: July 19, 2016

不同尺寸纳米塑料混合物的细胞吸收:相互作用和机制

Jie Liu¹, Yuan-Yuan Liu¹, Yuan Guo¹

¹Institute of Nanochemistry and Nanobiology, Shanghai University, Shanghai 200444, China.

Journal of hazardous materials

|February 28, 2026

概括

混合尺寸纳米塑料的细胞吸收不同于单一尺寸的纳米塑料. 较大的纳米塑料 (100纳米) 通过触发内细胞分裂来增强较小的纳米塑料 (50纳米) 的吸收,而较小的纳米塑料由于蛋白质冠变化而对较大的纳米塑料表现出复杂的影响.

科学领域:

环境科学环境科学
毒理学毒理学毒理学
细胞生物学细胞生物学

背景情况:

现实世界的纳米塑料具有不同的尺寸分布.
了解混合尺寸纳米塑料的细胞吸收机制对于评估它们对环境和健康的影响至关重要.

研究的目的:

在同时暴露条件下研究混合尺寸 (50nm和100nm) 聚乙烯纳米塑料的细胞吸收.
为了确定内细胞分裂机制是否在混合尺寸和单尺寸纳米塑料之间有所不同.

主要方法:

细胞同时暴露于50nm (G50PS) 和100nm (R100PS) 聚烯纳米塑料的不同度和比例.
使用流细胞计或类似技术分析细胞吸收.
研究内细胞形成途径,包括克拉特林介导的内细胞形成途径.
在含血清介质中的纳米塑料上蛋白质冠状形成的表征.

主要成果:

同时暴露100纳米纳米塑料 (R100PS) 一般会提高50纳米纳米塑料 (G50PS) 的细胞吸收率,高达74.2%.
R100PS通过触发克拉斯林介导的内细胞分裂来促进G50PS的吸收.
G50PS对R100PS吸收的影响是复杂的,根据混合条件和血清存在,显示了增强 (高达71.3%) 和抑制 (高达38.2%).
在含有血清的介质中,G50PS和R100PS之间的冠状蛋白竞争影响了R100PS的内细胞行为.

结论:

混合尺寸纳米塑料的细胞吸收不同于单一尺寸的纳米塑料,受粒子大小,度,比率和介质组成的影响.
纳米塑料与生物相互作用是复杂的,对内分细胞和蛋白质冠状形成的影响取决于大小.
这些发现强调了在纳米塑料毒性评估中需要考虑尺寸分布的必要性.

关键词:

共同暴露是指共同暴露.细胞内存症是指细胞内存症.纳米颗粒是如何形成的蛋白质王冠竞赛蛋白质王冠竞赛尺寸效应尺寸效应尺寸效应尺寸效应

更多相关视频

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Analyzing Cellular Internalization of Nanoparticles and Bacteria by Multi-spectral Imaging Flow Cytometry

Published on: June 8, 2012

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

Published on: September 2, 2022

相关概念视频

Factors Affecting Dissolution: Particle Size and Effective Surface Area

Factors Affecting Dissolution: Particle Size and Effective Surface Area

Dissolution kinetics, an essential aspect of oral drug delivery, is significantly influenced by the drug's particle size. According to the Noyes-Whitney dissolution model, the dissolution rate correlates directly with the drug's surface area. The larger the surface area, the higher the drug's solubility in water, leading to a faster drug dissolution rate. Reducing particle size increases the effective surface area, enhancing the dissolution process. Micronization and nanosizing are...

Mechanisms of Drug Absorption: Paracellular, Transcellular, and Vesicular Transport

Mechanisms of Drug Absorption: Paracellular, Transcellular, and Vesicular Transport

Drugs need to permeate cell membranes to reach their target sites after administration. Orally administered drugs must transcend intestinal epithelial membrane barriers to infiltrate the systemic circulation. Drugs with a molecular weight of less than 500 Daltons diffuse through gaps between neighboring cells, called paracellular pathways.
However, most drugs use the transcellular route, traversing directly through the cell membranes via two mechanisms: passive and active transport. Passive...