在羊体细胞核转移的关键步骤中应用RGFP966对克隆胚胎发育的影响
Hongyuan Song1, Zhipeng Qi1, Junli He1
1Key Laboratory of Ministry of Education for Conservation and Utilization of Special Biological Resources in the Western, Ningxia University, Yinchuan, Ningxia 750021, China; School of Life Sciences, Ningxia University, Yinchuan, Ningxia 750021, China.
Animal reproduction science
|February 28, 2026
概括
这项研究表明,RGFP966是一种基因组脱乙酶3 (HDAC3) 抑制剂,通过增强卵细胞成熟和胚胎发育来改善绵羊体细胞核转移 (SCNT). 然而,它也增加了细胞的亡,限制了胚胎细胞的发育率.
科学领域:
- 生殖生物学 生殖生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 发展生物学 发展生物学
背景情况:
- 羊体细胞核转移 (SCNT) 的效率受到早期胚胎发育问题的限制.
- 海斯脱乙酶3 (HDAC3) 参与胚胎发育,在被捕的绵羊胚胎中表达高.
研究的目的:
- 研究HDAC3选择性抑制剂RGFP966对优化绵羊SCNT的关键步骤的影响.
- 阐明RGFP966影响SCNT早期胚胎发育的机制.
主要方法:
- 采用了"18h + 4h"两步卵细胞成熟协议.
- 在卵细胞成熟,捐赠细胞预处理和重建胚胎培养期间,RGFP966在特定度下应用.
- 研究人员分析了表观遗传标记物 (H3K27ac,H3K9me3) 和肌体标记物 (CDX2).
- 评估了 morulae 中的亡水平.
主要成果:
- 在卵细胞成熟过程中,RGFP966显著增加了第一个极体挤出率.
- 结合RGFP966治疗改善了克隆胚胎分裂率,并增强了胚胎细胞表观遗传重编程 (增加H3K27ac,减少H3K9me3).
- 促进了托菲克多德的发育 (CDX2表达),但摩细胞的亡显著增加,限制了胚芽细胞的发育率.
结论:
- RGFP966联合治疗通过改善卵细胞成熟,表观遗传重编程和皮功能来优化早期羊克隆胚胎的发育.
- RGFP966诱导的细胞亡是改善绵羊SCNT中的胚芽细胞率的限制因素.
- 有针对性的HDAC3监管为提高SCNT效率提供了理论基础.
相关概念视频
01:08Cloning of Dolly the Sheep
8.2K
The first successfully cloned mammal was Dolly, a sheep, born on 5th July 1996 at Roslin Institute, Scotland. The cloned sheep was named after the American singer Dolly Parton. Dolly lived for seven years and died of respiratory complications, which is speculated to be due to the actual age of her DNA. Because the DNA in cloned cells belongs to an older individual, the cloned individual’s life expectancy may be affected. Indeed, analysis of Dolly’s DNA revealed shorter...
8.2K
01:27Reproductive Cloning
32.9K
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
32.9K
01:14Introduction to Nuclear Reprogramming
2.3K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.3K
01:24Methods of Nuclear Reprogramming
2.2K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.2K