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Updated: Mar 2, 2026

Cryogenic Sample Loading into a Magic Angle Spinning Nuclear Magnetic Resonance Spectrometer that Preserves Cellular Viability
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准备和表征样本的协议,用于微秒结合结-超衰减和电子偏磁共振光谱学的样本

Joshua L Wort1, Tobias Hett1, Adrian Haardt1

  • 1Clausius Institute for Physical and Theoretical Chemistry, Rheinische Friedrich-Wilhelms-Universität Bonn, Wegelerstraße 12, 53115 Bonn, Germany.

STAR protocols
|February 28, 2026
PubMed
概括

这项研究详细介绍了一项使用微秒结超振动 (MHQ) 和脉冲电子-电子双共振 (PELDOR) 谱学的协议,以分析生物分子构造变化和蛋白质-连接体相互作用.

关键词:
生物物理学的生物物理.蛋白质生物化学 蛋白质生物化学蛋白质的表达和净化过程结构生物学 结构生物学

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Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
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