带血衍生自然杀手细胞作为采用细胞治疗的可行和强有力的来源
Raquel de Melo Alves-Paiva1, Larissa L Coa1, Julia T Azevedo1
1Department of Hemotherapy and Cell Therapy, Einstein Hospital Israelita, São Paulo, Brazil.
Cytotherapy
|February 28, 2026
概括
由带血 (UCB) 衍生的自然杀手 (NK) 细胞可以被活体扩展和冷保存. 这些冷保存的NK细胞保持着高活力和强大的细胞毒性活性,支持它们在采用免疫疗法中的使用.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞疗法细胞疗法
- 癌症研究 癌症研究
背景情况:
- 自然杀手 (NK) 细胞对抗瘤和感染的天生的免疫力至关重要.
- 带血 (UCB) 是NK细胞的可获得来源,可降低异构移植风险.
研究的目的:
- 评估扩张和冷保存UCB衍生的NK细胞的可行性.
- 评估冷保存的NK细胞的活力,免疫类型和细胞毒性功能.
主要方法:
- 在GMP条件下,使用CSTX002在体外扩展14天的UCB衍生NK细胞.
- 活力,免疫类型 (CD56+CD3-),以及针对白血病细胞系 (K562,HL-60) 的细胞毒性活动在扩张后和冷保存后进行了评估.
主要成果:
- 扩张的NK细胞显示出高活力 (94.8%),并在冷保存365天 (91%) 后保持高活力.
- 在长期冷保存后,NK细胞免疫类型被保存 (>90%).
- 冷保存的NK细胞对白血病点保持了强大的细胞毒性活性,与新鲜细胞相比.
结论:
- 由UCB衍生的冷保存的NK细胞是采用免疫治疗的稳定有效的"现成"产品.
- 这种方法在可扩展性,标准化和快速临床应用方面具有优势,特别是在急性髓性白血病方面.
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