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牛奶蛋白衍生的免疫调节:酶制剂,序列功能映射和具有免疫相关活动的抗氧化能力

Meng-Qi Liu¹, Ying-Chao Qiu¹, Hong-Fu Zhao¹

¹Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin, 150030, PR China; Department of Food Science, Northeast Agricultural University, Harbin, 150030, PR China.

International journal of biological macromolecules

|February 28, 2026

概括

牛奶蛋白质水解剂产生多功能,具有抗氧化和免疫增强的特性. 来自alpha-lactalbumin的特定可以增强免疫细胞功能和氧化还原平衡.

科学领域:

食品科学与技术食品科学与技术
生物化学生物化学
免疫学免疫学免疫学

背景情况:

牛奶蛋白是生物活性的丰富来源.
生物活性可以表现出各种促进健康的特性,包括抗氧化和免疫调节作用.
了解结构和功能之间的关系对于开发功能性食品至关重要.

研究的目的:

从牛奶蛋白中生成和表征多功能.
研究这些的免疫调节和抗氧化活性.
通过分子对接来探索它们生物活性的潜在机制.

主要方法:

使用性蛋白酶或素-素的α-乳蛋白,β-乳蛋白和素的水解.
用LC-MS/MS进行水解酸盐的分化和表征.
在体外测试抗氧化能力和免疫调节活性 (细胞,促炎媒介分泌).
分子对接模拟用于预测-标相互作用 (TLR2/TLR4,Keap1).

主要成果:

来自α-乳酸蛋白的片 (F2) 呈现出最高的抗氧化能力.
来自F2的三种 (GRFF,WDSF,SVPGPMGPSGPR) 在RAW264.7细胞中增强了细胞形成和促炎媒介分泌.
这些也表现出显著的体外抗氧化能力.
分子对接表明与Toll类受体 (TLR2 / TLR4) 和Keap1的相互作用,将免疫激活与氧化还原调节联系起来.

结论:

具有综合免疫调节和抗氧化特性的多功能生物活性可以从牛奶蛋白中提取.
来自α-乳蛋白解液的F2分量含有功能性食品应用的有希望的候选.
这项研究提供了对这些牛奶衍生的双重生物活性的机制性见解.

关键词:

抗氧化能力的抗氧化能力.免疫调节活性免疫调节活性牛奶中的蛋白质来源于牛奶.

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