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整合性星际-DIA蛋白质组和转录组揭示了类植物Hordeum marinum中的候选盐耐受性基因

Hongxing Zhou¹, Boyang Zhang¹, Lin Li¹

¹College of Agronomy, Hunan Agricultural University, Changsha 410128, China; Yuelushan Laboratory, Changsha 410128, China.

Journal of proteomics

|February 28, 2026

概括

海大麦草 (Hordeum marinum) 通过联合离子稳态和抗氧化剂防御来保持盐分耐受性. 这项研究确定了开发耐盐作物的关键基因和途径.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

土壤盐化威胁着全球农业.
像海大麦草 (Hordeum marinum) 这样的状物种具有特殊的盐分耐受性.
了解这种耐受性的分子基础对于作物改进至关重要.

研究的目的:

为了阐明海洋大麦草 (Hordeum marinum) 极端耐盐的分子遗传基础.
为了确定关键的蛋白质和途径参与离子稳态和抗氧化防御在盐的压力下.

主要方法:

在NACl处理下对Hordeum marinum的根样进行高分辨率定量蛋白质组学 (Astral-DIA).
与根转录组概况的集成.
凯格丰富分析和亚细胞局部化分析.

主要成果:

观察到与碳代谢,信号通路 (MAPK,) 和蛋白质加工相关的蛋白质丰度的显著变化.
关键的离子载体 (SOS1,V-ATPase,KEA2) 显示出对Na+挤出和K+吸收的协调活动.
谷氨代谢途径在蛋白质组和转录组数据中都得到了融合的丰富,这表明它在氧化还原稳定中的作用.

结论:

马林通过协同的离子恒温和抗氧化剂防御保持低的Na+/K+比.
这一策略是其优越的盐分耐受性的关键决定因素.
已识别的候选基因和通路可用于设计耐盐谷物作物.

关键词:

星际-DIAIA是什么意思谷氨的新陈代谢荷尔代姆马里纳姆 (Hordeum marinum) 是一个海洋的群体.离子运输离子运输蛋白质组学是指蛋白质组学.盐的压力是盐的压力.

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