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粘附和悬浮肉纤维细胞系的比较转录组学,用于优化培养肉类加工

Elizabeth J Contreras¹, Archana Nagarajan¹, Benjamin H Bromberg²

¹Department of Biomedical Engineering, Tufts University School of Engineering, Medford, MA 02155, USA.

Food research international (Ottawa, Ont.)

|February 28, 2026

概括

细胞通过调节新陈代谢和细胞结合来适应悬浮培养,揭示了一种涉及MAPK p38δ,TEAD,YAP和FoxO1的新机制,用于培养肉类生产.

科学领域:

生物技术是生物技术.
细胞生物学细胞生物学
食品科学食品科学食品科学

背景情况:

培养肉类行业需要能够在悬浮中生长的细胞系,以实现高效的生物质生产.
目前动物细胞适应悬浮培养的局限性阻碍了经济高效的培养肉类开发.

研究的目的:

研究胚胎纤维细胞 (DF-1细胞) 适应悬浮培养的分子机制.
确定参与实现培养肉应用悬浮能力的关键监管机构.

主要方法:

利用时间序列RNA测序来分析DF-1细胞在从附着到悬浮培养的过渡期间的转录变化.
研究了基激活蛋白激酶 (MAPK) p38δ,TEA域 (TEAD) 蛋白,YES相关蛋白 (YAP) 和叉头盒蛋白1 (FoxO1) 在悬浮适应中的作用.

主要成果:

DF-1细胞在应对氧化应激和脱离时降低非基本代谢,调节细胞结节.
发现了一种新的机制,其中MAPK p38δ将TEAD蛋白质隔离,促进YAP-FoxO1相互作用,导致细胞矩阵解离和抗氧化剂产生.

结论:

这项研究揭示了在细胞中实现悬浮能力的新途径,这对于培养肉类生产至关重要.
这些发现为开发新的悬浮适应细胞系为更广泛的食品相关细胞工业提供了基础.

关键词:

肉肉肉肉培养肉类的培养肉.培养肉类的培养肉.纤维母细胞细胞是如何形成的没有加入的非成员.暂停暂停暂停

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