无活化机制和参数优化氧低压冷血对悬浮和奶酪上的食物传播病原体
Rihua Xu1, Geqi Qi1, Hai Zhang2
1School of Life Science, Inner Mongolia University, Hohhot 010070, China.
Food research international (Ottawa, Ont.)
|February 28, 2026
概括
/氧低压冷等离子体 (LPCP) 有效地杀死食品表面上的大肠杆菌等细菌. 这种非热方法破坏细菌膜,增强食品安全,而不会显著改变味道或质地.
科学领域:
- 食品科学与技术 食品科学与技术
- 微生物学 微生物学
- 等离子体物理学的物理学
背景情况:
- 食品传播的病原体,如大肠杆菌,黄金杆菌和单细胞杆菌,构成重大风险.
- 传统的消毒方法可能很苛刻,影响食品质量.
- 非热等离子体为食品表面脱污染提供了一个潜在的替代方案.
研究的目的:
- 量化/氧低压冷血 (Ar/O2 LPCP) 对关键的食源性病原体的杀菌效果.
- 研究Ar/O2 LPCP对细菌膜完整性和食品微观结构的影响.
- 优化Ar/O2 LPCP处理参数,以实现有效和安全的食品表面净化.
主要方法:
- 用Ar/O2 LPCP处理细菌悬浮和奶酪表面.
- 使用殖民地形成单位数量的细菌减少量化.
- 细菌膜损伤的分析通过马隆迪甲基和蛋白质泄漏测定,电子显微镜,里埃变换红外光谱学和流细胞计.
- 使用响应表面方法和在奶酪表面的验证进行优化.
- 使用显微镜和感官评估评估奶酪微观结构和感官特性.
主要成果:
- 短时间的Ar/O2LPCP治疗 (1分钟) 显著减少了悬浮中的大肠杆菌,黄金杆菌和单细胞杆菌 (>2.4 log CFU/ml).
- 通过增加的泄漏标记和细胞结构损伤,细菌膜的破坏显而易见.
- 在奶酪表面的优化Ar/O2LPCP处理实现了显著的细菌减少 (1.171.64日志CFU/g),在短时间暴露 (<1分钟) 时对微观结构的影响最小.
- 较长的暴露时间 (>1.67分钟) 导致脂质氧化增加和感官属性 (气味,质地) 降低.
结论:
- Ar/O2 LPCP是一种高效的非热方法,用于减少食品表面的细菌污染.
- 主要机制涉及细菌膜完整性的破坏.
- 优化,短时间的Ar/O2 LPCP治疗可以提高食品安全,而不会影响感官质量.
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