双重微管相关的 tektins 和酶差异性调节精子鞭毛完整性和运动性
Qi Liu1, Lunni Zhou2,3,4,5, Xiaochen Liang2,3,4,5
1Department of Obstetrics and Gynecology, Sir Run Run Shaw Hospital and Liangzhu Laboratory, Zhejiang University School of Medicine, Hangzhou, Zhejiang, China.
Nature communications
|March 1, 2026
概括
双微管 (DMT) 相关的蛋白质对于精子运动和受精至关重要. 这项研究揭示了特定的DMT蛋白,如TEKT1和TSSK6,如何影响精子功能,为男性不孕症的原因提供了洞察力.
科学领域:
- 精子生物学和男性生殖健康.
- 鞭毛组合和功能的分子机制.
- 遗传学和蛋白质在受精过程中的作用.
背景情况:
- 双微管 (DMT) 相关的蛋白质对于精子鞭毛的组装和功能至关重要.
- 单个DMT蛋白及其精子中的分子机制的确切作用尚未完全理解.
- 了解这些蛋白质是诊断和治疗男性不孕症的关键.
研究的目的:
- 为了研究精子中不同的DMT相关蛋白 (TEKT1,TEKT5,TSSK6,DUSP21) 的特定功能.
- 阐明它们在精子运动和受精中的作用背后的分子机制.
- 探索对诊断男性不孕症的影响.
主要方法:
- 对TEKT1,TEKT5,TSSK6和DUSP21.21进行基因淘汰的小鼠的生成.
- 对DMT组件的高分辨率结构分析.
- 在淘汰赛模型中评估精子运动,形态和生育能力.
- 蛋白组学用于分析蛋白质酸化变化.
主要成果:
- 由于TEKT1的耗尽,导致男性不育,精子运动能力受损,并失去TEKT1束.
- Tekt5淘汰赛小鼠表现出正常的生育能力,这表明了tektin家族内的功能分歧.
- 绝杀精子表现出严重的形态和运动缺陷,导致不孕.
- 在Tssk6 KO小鼠中观察到对轴膜蛋白质的失调酸化.
- Dusp21淘汰赛小鼠没有生育或运动缺陷,这表明了补偿机制.
结论:
- 丝状 (TEKT1) 和酶性 (TSSK6) DMT 蛋白通过不同的机制控制精子功能.
- TEKT1和TSSK6在精子运动和男性生育能力方面发挥着关键的,非冗余的作用.
- 这些发现对精和男性不孕症的分子诊断有意义.
相关概念视频
Microtubules in Cell Motility
4.9K
Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
4.9K
Destabilization of Microtubules
3.8K
The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...
3.8K
Microtubule Associated Motor Proteins
11.1K
Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular...
11.1K
Microtubule Instability
6.3K
Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...
6.3K
Microtubules
102.1K
There are three types of cytoskeletal structures in eukaryotic cells—microfilaments, intermediate filaments, and microtubules. With a diameter of about 25 nm, microtubules are the thickest of these fibers. Microtubules carry out a variety of functions that include cell structure and support, transport of organelles, cell motility (movement), and the separation of chromosomes during cell division.
102.1K
Microtubules
11.1K
Microtubules are the thickest cytoskeletal filaments with a diameter of 25 nm. In prokaryotic organisms, microtubules are commonly found in locomotory appendages like cilia and flagella. In eukaryotic cells, microtubules form specialized extensions for moving fluid over the surface, like those found in cells lining the intestine.
Microtubules have two structurally similar globular protein subunits: α and β tubulins. In the cytosol, the α and β tubulins form a heterodimer....
Microtubules have two structurally similar globular protein subunits: α and β tubulins. In the cytosol, the α and β tubulins form a heterodimer....
11.1K


