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在饮食纤维分析中的染色学考虑和马尔蒂托尔干扰

Tadas Kargelis¹, Zhen K Lam¹, David Mangan¹

¹Neogen, Bray Business Park, Southern Cross Rd, Bray, Co. Wicklow, Ireland (A98 YV29).

Journal of AOAC International

|March 1, 2026

概括

在HPLC中选择的列影响了全食纤维 (TDF) 分析. 基于粉的食品中的马尔蒂托尔可以错误地提高可溶性食纤维 (SDFS) 的测量值,需要改进方法.

科学领域:

分析化学分析化学
食品科学食品科学食品科学
生物化学生物化学

背景情况:

AOAC 方法 2017.16 和 2022.01 是总食纤维 (TDF) 分析的标准程序.
这些方法使用酶-重力测量步骤和HPLC用于可溶性食纤维 (SDFS) 的量化.
染色学和矩阵干扰的局限性可能会影响SDFS的准确性.

研究的目的:

在TDF分析中评估HPLC列,以准确地分配DP2/DP3边界.
调查马尔蒂托尔干扰导致SDFS在粉食品中的高估.

主要方法:

在五个HPLC列配置中分析了74个碳水化合物标准.
使用缩放的AOAC方法评估了食品矩阵中的马尔蒂托尔干扰.
基于与马尔分界相对的峰值区域分配得分的列表表现.

主要成果:

糖包 (水) 列系列显示DP2/DP3分配的最高准确度.
通过调整DP2/DP3划分,可以单独排除马耳他醇.
麦芽醇与葡萄糖聚合物导致小麦粉混合物中~8%的明显SDFS高估.

结论:

HPLC 列选择显著影响 SDFS 量化准确性.
马尔蒂托尔可以导致人工SDFS粉含量样本由于酶处理.
对于强大的AOAC食纤维方法,需要进一步研究和指导列等价性.

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