富含多糖的Lycium barbarum提取物 通过肠道微生物群介导的Tfh/B细胞轴调节改善类风湿性关节炎
Chunlei Jiang1, Danyan Chen1, Jing Li1
1Department of Pathogen Biology and Immunology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia, China.
概括
利巴巴鲁姆多糖提取物 (LBP) 调节了类风湿性关节炎 (RA) 患者和CIA老鼠的肠道微生物群. 低血压治疗减少了T毛囊辅助细胞 (Tfh) 和B细胞激活,改善了RA病理.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物组研究的研究.
- 类风湿病学 类风湿病学
背景情况:
- 肠道微生物群失调与类风湿性关节炎 (RA) 有关.
- 富含Lycium barbarum多糖提取物 (LBP) 具有抗炎性质.
- 细菌驱动因素和RA的机制,特别是T卵泡辅助细胞 (Tfh) 细胞/B细胞轴,尚未完全理解.
研究的目的:
- 调查RA患者和原诱导性关节炎 (CIA) 鼠标模型中的肠道微生物群变化.
- 通过评估其对肠道微生物群的影响,评估LBP在RA中的治疗潜力.
主要方法:
- 16S rRNA基因测序用于RA患者和CIA老鼠的肠道微生物群分析.
- 流细胞计,ELISA和HE染色以探索LBP的作用机制.
- 评估Tfh细胞,B细胞,血清细胞因子和关节/结肠组织病理.
主要成果:
- 关节炎患者表现出肠道微生物多样性的减少,Ligilactobacillus和Escherichia-Shigella的减少,Blautia的增加.
- 在CIA老鼠中进行的LBP治疗恢复了Ligilactobacillus和Limosilactobacillus,同时减少了Blautia.
- 脊髓Tfh和B细胞减少,血清IgG,IL-6和IL-21降低,并改善关节炎症和结肠损伤.
结论:
- 肠道功能失调和Tfh细胞激活对于RA的发展和进展至关重要.
- 利吉拉ctobacillus和Blautia与人类和CIA老鼠的RA病理有关.
- 低血压可能抑制Tfh细胞增殖和B细胞分化,通过调节异常免疫反应,为RA提供治疗途径.
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