由FAdV-4诱导的二次巴氏菌多种菌感染增强了细菌的附着性,并加剧了SPF的病理损伤
Yuxin Chen1, Guizhen Yi2, Yunzhen Huang3
1College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China; Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangdong Province Key Laboratory of Livestock Disease Prevention, Guangdong Province Key Laboratory for prevention and control of Avian Influenza and Other Major Poultry Diseases, Ministry of Agriculture and Rural Affairs, 510640, China.
Veterinary microbiology
|March 1, 2026
概括
禽类亚维亚代诺病毒-4 (FAdV-4) 和帕斯图雷拉多菌 (PM) 的联合感染会导致的严重疾病. FAdV-4增强PM殖民和肝损伤,TLR4信号在这种协同效应中发挥着关键作用.
科学领域:
- 兽医病毒学 兽医病毒学
- 鸟类病理学 鸟类病理学
- 免疫学 免疫学 免疫学
背景情况:
- 禽类禽类腺病毒 (FAdV) 和多种菌 (PM) 是常见的家禽病原体.
- 联合感染可能会导致比单一感染更严重的疾病,但相互作用尚未得到充分理解.
研究的目的:
- 为了研究FAdV-4和PM共感染在中的协同致病作用.
- 阐明潜在的分子机制,特别是托尔类受体4 (TLR4) 的作用.
主要方法:
- 使用无特定病原体 (SPF) 建立了一个体内模型,以研究FAdV-4二次PM感染.
- 对肝脏组织进行了转录组测序,以分析基因表达变化.
- 使用 leghorn雄性肝细胞 (LMH) 细胞的体外模型被用来评估病原体的粘附和入侵.
- 使用TLR4激动剂 (LPS) 和抑制剂 (Resatorvid/TAK-242) 来操纵TLR4表达.
主要成果:
- FAdV-4感染显著增加了PM殖民,导致临床症状加剧和肝损伤.
- 同时感染的的血清细胞因子水平 (TNF-α,CCL-4,IL-6,IL-1β) 升高.
- 转录组分析显示,炎症反应基因的上调,包括IL6和TLR4.
- 在体外,FAdV-4促进了PM粘附和入侵,与增加TLR4表达有关.
- 调节TLR4表达直接影响了PM粘附和入侵.
结论:
- 在中,FAdV-4和PM具有协同的致病作用.
- 收费类受体4 (TLR4) 途径在FAdV-4和PM共感染期间调解增强的致病性方面发挥着关键作用.
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