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通过3D前列腺球体和瘤流体相互作用模型中的炎症微环境对与EMT相关的路径的差异调节

Fersu G A Calisir¹, Suleyman Arziman², Elif Isel²

¹Ege University, Faculty of Pharmacy, Department of Pharmaceutical Biotechnology, Izmir, Turkiye.

Experimental cell research

|March 1, 2026

概括

炎症驱动前列腺癌 (PCa) 的进展,通过促进上皮层 - 介质细胞过渡 (EMT). 优化的3D模型揭示了瘤-肌瘤相互作用极大地影响炎症诱导的EMT和PCa转移,有助于向治疗的开发.

科学领域:

在瘤学瘤学.
细胞生物学细胞生物学
癌症研究癌症研究

背景情况:

慢性炎症是前列腺癌 (PCa) 发病,进展和转移的关键驱动因素.
炎症促进瘤发生过程,如上皮层-介质细胞过渡 (EMT).

研究的目的:

在PCa中优化体外模型来研究炎症驱动的EMT.
为了研究瘤-肌瘤相互作用在炎症诱导的PCa进展中的作用.

主要方法:

开发和优化3D前列腺癌球体.
在炎症条件下建立上皮质-肌纤维细胞共同培养系统.
对EMT介导体和细胞行为对炎症刺激的反应进行分析.

主要成果:

球状体的形成取决于细胞系,而不仅仅取决于大小.
炎症刺激在3D球体中不同调节EMT介质 (纤维素,维门丁,TWIST1),增强侵入性特征.
共同培养模型表明,树叶的影响会影响炎症诱导的阿诺基克的抗性和细胞迁移.

结论:

在PCa中,瘤-肌瘤相互作用对于调解炎症对EMT的影响至关重要.
优化的3D球形和共同培养模型为研究PCa进展机制提供了一个强大的平台.
这些发现支持开发针对PCa的有针对性的治疗策略.

关键词:

前列腺癌的球形形状.表皮细胞-介质细胞过渡.长皮质-肌纤维细胞共同培养炎症诱导的瘤发生.瘤-肌瘤相互作用

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