用XGBoost优化的提取性共发酵及其宏分子特征的方法,可持续地将农业残留物价值化为聚基酸和氨酸
Gracia Saral Gladison1, Lavanya Priyadarshini Ramalingam2, Udayakumar Mani1
1Green Separation Engineering Laboratory, SASTRA Deemed University, Thanjavur, Tamil Nadu, India.
International journal of biological macromolecules
|March 1, 2026
概括
农业废物通过提取性共发酵转化为有价值的聚乙酸 (PHB) 和氨酶酶. 这种可持续的方法回收废物,支持循环生物经济,并与气候行动目标保持一致.
科学领域:
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
- 可持续化学 可持续化学
背景情况:
- 全球粮食生产产生大量的农业垃圾,这给环境带来了挑战.
- 通过生物工艺对农业废物进行价值化,为处置提供了一个可持续的替代方案.
- 聚基酸盐 (PHB) 和酶是有价值的生物制品,具有多种应用.
研究的目的:
- 从农业废物中开发一种高效和具有成本效益的方法来生产聚基酸盐 (PHB) 和氨酶酶.
- 使用大米和玉米优化提取性共发酵过程.
- 描述产生的PHB和酶,并验证回收过程.
主要方法:
- 采用Priestia megaterium的米和玉米的提取性共发酵.
- 优化化时间和媒介组成,以提高产量.
- 响应表面方法 (RSM) 和机器学习 (XGBoost) 的应用用于过程建模和预测.
- 使用FTIR,GC-MS,HPLC,NMR和GPC进行PHB的表征.
- 通过凝过色谱来净化酶.
主要成果:
- 在48小时化后,获得PHB (83.10%) 和氨酶 (81.37%) 的高产.
- 使用PEG度,盐度和温度 (30°C) 的优化酶恢复,预测准确率为95% (XGBoost).
- RSM模型显示了酶和PHB生产的高相关性 (R2=0.96).
- PHB的表征证实了它的身份和纯度.
- 在净化后,酶回收率为83.45%.
结论:
- 基于PEG的提取性共发酵是一种高效且负担得起的方法,用于从农业废物中生产PHB和酶.
- 这种方法通过将废物转化为有价值的产品来支持循环生物经济.
- 该研究与可持续发展目标 (SDG) 12 (负责任的消费和生产) 和SDG 13 (气候行动) 保持一致.
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