一种高效的性酸酶,用于对宏分子基质进行脱
Yu Xie1, Xingyu Shen2, Zengxing Yi3
1State Key Laboratory of Green Biomanufacturing, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China; Jiangxi Provincial Engineering Research Center for Recycling Technology of Traditional Chinese Medicine Herbal Residue, College of Pharmacy, Yichun University, Yichun, 336000, China.
International journal of biological macromolecules
|March 1, 2026
概括
酵母 (sAP) 中的一种新型性酸酶有效地从脂多糖 (LPS) 和蛋白质等复杂分子中去除基. 这种酶,sAP,与小牛肠酸酶 (CIP) 相比,显示出更高的去酸化活性,并中和内毒素活性.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 酶学 是一种酶学.
背景情况:
- 含的宏分子在细菌病原和蛋白质修饰中至关重要.
- 牛犊肠性酸酶 (CIP) 在去化复杂生物大分子,如脂多糖 (LPS) 和蛋白质方面具有有限的有效性.
研究的目的:
- 来自Saccharomyces cerevisiae (sAP) 的性酸酶的表达和特征,用于增强宏分子基质的脱化.
- 为了评估与CIP相比重组sAP (rsAP) 在生物技术和治疗应用中的有效性.
主要方法:
- 再组合的sAP (rsAP) 被表达为Komagataella phaffii.
- 评估了酶活性,在各种pH值和温度范围内的稳定性.
- 对LPS,酸化蛋白和DNA的脱酸化效率与CIP进行了比较.
- 在中和内毒素活性和在小鼠内毒素模型中的有效性被评估.
主要成果:
- rsAP成功表达,产量为1.12g/L.
- rsAP在pH10.0和65°C时表现出最佳活性,具有广泛的稳定性.
- 与CIP相比,rsAP对LPS,酸化蛋白和DNA的脱酸化效率显著更高.
- rsAP有效地中和了内毒素活性,在小鼠内毒素病模型中提高了生存率.
结论:
- 与CIP相比,rsAP是一种强大的生物催化剂,与复杂的生物宏分子相比,具有更高的脱化活性.
- rsAP显示了LPS排毒和蛋白修饰的显著潜力.
- rsAP的独特结构特征有助于提高其催化效率,使其成为生物技术和治疗应用的有价值工具.
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