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综合性和结合性元素的表征携带 bla_NDM-1和 bla_KPC-2在环境中的Pseudomonas guariconensis隔离物中

Duc Trung Dao¹, Masato Suzuki^2,3, Yohei Kobayashi^1,4

¹Department of Bacteriology, Nagoya University Graduate School of Medicine, Japan.

Japanese journal of infectious diseases

|March 1, 2026

概括

城市废水含有抗生素耐药性基因. 研究人员在Pseudomonas菌株中发现了两种携带碳烯抗性基因 (blaNDM-1和blaKPC-2) 的移动遗传元素,突出了环境细菌.

科学领域:

环境微生物学环境微生物学
分子生物学分子生物学
抗微生物耐药性研究的研究.

背景情况:

城市废水是抗菌素耐药性 (AMR) 和水平基因转移的重要储备.
环境中多药耐药细菌的日益流行构成了全球健康威胁.

研究的目的:

在越南河内,从城市废水中分离出来的细菌中研究碳烯耐药性的存在和机制.
描述负责耐药性基因传播的移动遗传元素.

主要方法:

从城市废水中分离和识别多药耐药细菌.
全基因组测序以识别耐药性基因和移动遗传元素.
使用PCR和测序确认整合和结合元件 (ICE) 切除和循环化.
细菌结合实验,以评估基因转移效率.

主要成果:

隔离了一种耐多药性Pseudomonas guariconensis菌株 (KNHN1),该菌株对卡巴烯和虫素具有耐药性.
鉴定了两种新的染色体集成ICE,ICEPgKNHN1_KPC和ICEPgKNHN1_NDM,分别携带blaKPC-2和blaNDM-1.
证明ICE切除,循环化和对受体菌株的有效结合,从而赋予广泛的β-乳抗性.

结论:

这项研究报告了P. guariconensis中第一个染色体集成的blaNDM-1和blaKPC-2的实例,由功能性ICE介导.
环境细菌,特别是城市废水中的细菌,作为临床上重要的抗生素耐药性基因的关键储存库.
整合性和结合性元素是碳烯耐药性在环境传播的关键驱动因素.

关键词:

伪虫 (Pseudomonas guariconensis) 是一种传染病.抗微生物耐药性抗微生物耐药性在blaKPC-2中使用.这就是blaNDM-1的原因.整合和结合元素的整合和结合元素.

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