在人体细胞中,三酸诱导的细胞损伤是通过内细胞网膜应激引起的
Hejun Duan1, Liyu Huang2, Yixing Feng1,3
1Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Control and Prevention, China.
The Journal of toxicological sciences
|March 1, 2026
概括
酸三 (TPhP) 暴露会通过诱导内质网膜 (ER) 应激而损害细胞. 一种ER压力抑制剂,4-基酸 (4-PBA),有效地保护细胞免受TPhP毒性的影响.
科学领域:
- 毒理学 毒理学 毒理学
- 细胞生物学 细胞生物学
- 环境健康 环境健康
背景情况:
- 三酸 (TPhP) 是一种广泛使用的有机阻燃剂 (OPFR).
- 暴露于TPhP与毒性有关,而内质网膜 (ER) 压力与污染物诱导的病有关.
- 在TPhP诱导的损伤中,ER压力的具体作用尚不清楚.
研究的目的:
- 研究TPhP对人类管状上皮细胞 (HKC) 的影响.
- 为了确定ER压力的参与TPhP诱导的毒性.
- 评估ER应激抑制对TPhP损伤的保护潜力.
主要方法:
- HKC细胞暴露于不同度的TPhP.
- 评估了细胞活力,细胞亡 (Caspase-3激活) 和ER压力标志物.
- 细胞与TPhP和4-甲酸 (4-PBA),一种ER压力抑制剂进行了联合治疗.
主要成果:
- TPhP以剂量依赖的方式显著抑制HKC细胞活力 (48小时后LC50 = 126.4μM).
- TPhP诱导了度依赖性亡和上调的ER与压力相关的基因.
- 4-PBA治疗减弱了TPhP诱导的ER压力和HKC细胞中的细胞毒性.
结论:
- 细胞内膜网膜 (ER) 的压力在TPhP诱导的细胞损伤中起着至关重要的作用.
- 4-基酸盐 (4-PBA) 显示出作为治疗剂的潜力,可以减轻TPhP毒性.
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