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Updated: Mar 3, 2026

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开发和验证直接殖民地实时PCR用于快速和特定的Candida auris检测的开发和验证

Takashi Umeyama1, Tsuguto Fujimoto1, Jun Ishikawa1

  • 1Department of Fungal Infection, National Institute of Infectious Diseases, Japan Institute for Health Security.

Medical mycology journal
|March 1, 2026
PubMed
概括

一种新的实时PCR测定可以快速识别Candida auris,一种多抗药性真菌,直接来自没有DNA提取的殖民地. 这一突破有助于及时诊断和控制这种新兴病原体的感染.

关键词:
它们是Candida auris.直接殖民地PCR进行了PCR.设计初级设计初级设计快速检测 快速检测 迅速检测在实时PCR中进行实时PCR.

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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