相关实验视频

Updated: Mar 3, 2026

Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays
08:27

Visualization and Quantification of Endogenous Intra-Organelle Protein Interactions at ER-Mitochondria Contact Sites by Proximity Ligation Assays

Published on: October 20, 2023

2.5K

使用依赖于二分化的光蛋白来成像器官间膜接触点

Victoria H Williams1, Chih-Hsuan Hsu1, Gregory E Miner1

  • 1Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, United States.

Methods in enzymology
|March 1, 2026
PubMed
概括

膜接触部位 (MCSs) 对于细胞功能至关重要,但很难研究. 该协议使用新的Contact-FP生物传感器,使用先进的显微镜技术可视化活细胞中的MCS.

关键词:
生物传感器是一种生物传感器.细胞内膜网膜是什么 细胞内膜网膜光蛋白质是一种光蛋白质.脂质的滴滴,就是脂质滴滴.Lysosomes 是一个溶解体.膜接触部位与膜的接触部位.线粒体中的线粒体.有机器人有机器人过氧化物体是多氧化的血膜中的等离子体膜.

更多相关视频

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy08:55

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

10.2K
Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts — Implications for Neurodegenerative Disease Pathogenesis09:09

Author Spotlight: Regulation and Dysregulation of ER-Mitochondria Contacts — Implications for Neurodegenerative Disease Pathogenesis

Published on: October 11, 2024

3.1K

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K