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解读内生菌Trichoderma atroviride D16中耐受性背后的机制

Xue-Wen Bi¹, Xing-Guang Xie², Wen-Jing Zhang¹

¹School of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, 311402, Zhejiang, PR China.

|March 1, 2026

概括

内生真菌Trichoderma atroviride D16对 (Cd(II)) 具有很高的耐受性,并积极增强植物的耐药性. 这种真菌为减轻环境中的污染提供了一个有希望的,经济的解决方案.

科学领域:

环境微生物学环境微生物学
生物修复是一种生物修复.
植物与真菌的相互作用

背景情况:

重金属污染,特别是 (Cd) 污染,由于其持久性和毒性,造成了重大环境和健康风险.
植物内菌是开发可持续和成本效益高的生物修复策略的大量未开发资源.

研究的目的:

评估从Salvia miltiorrhiza中分离的内菌Trichoderma atroviride D16的Cd(II) 耐受性和缓解潜力.
阐明D16的Cd (II) 耐药性及其增强植物耐受性的分子机制.

主要方法:

在不同的Cd (II) 度下评估真菌生长和耐受性指数.
确定Cd (II) 吸附效率和EC50值.
在Cd(II) 压力下分析基因表达 (SOD,GPX,GSH) 和转录组概况.
在Cd (II) 暴露下评估D16注射对Arabidopsis thaliana的影响.

主要成果:

特里科德玛阿特罗维里德 (Trichoderma atroviride D16) 具有较高的Cd (II) 耐受性 (EC50=47.21毫克L-1),在高达150毫克L-1.1的度下对生长的影响最小.
在压力下,D16表现出显著的Cd(II) 吸附 (20.937.6%) 和高调的抗氧化酶 (SOD,GPX,GSH) 和转运基因.
用D16注射增加了Arabidopsis生物量,减少了Cd (II) 积累,表明宿主植物耐受性得到改善.

结论:

特里科德玛D16具有强大的内在耐药性对Cd (II) 并积极赋予宿主植物增强的耐受性.
D16通过增强宿主抗氧化防御和调节Cd-响应基因来控制金属吸收和促进排毒来减轻Cd (II) 压力.
这项研究为污染环境的生物修复提供了关键的微生物资源和理论支持.

关键词:

抗氧化剂活性抗氧化剂活性是的组成部分.葡萄糖氨酸 (Glutathione) 是一种转录组转录组就是一个转录组.三皮菌的阿特罗维里德D16 D16

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