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血液溶解对生物化学测试的影响:确定干扰截止值和管理血液溶解样本

Babak Shirazi Yeganeh¹, Neda Soleimani^1,2, Saeedeh Zare¹

¹Department of Pathology, Shiraz Medical School, Shiraz University of Medical Sciences, Shiraz, Iran, sums.ac.ir.

Journal of analytical methods in chemistry

|March 2, 2026

概括

在生物化学测试中,血液溶解的干扰会导致错误. 建议血解指数 (HIX) 截止值为0.5,以安全报告轻度血解样本,尽量减少诊断不准确性.

科学领域:

临床化学临床化学
实验室医学实验室医学
分析生物化学分析生物化学

背景情况:

血液溶解是生物化学测试中的常见干扰,可能导致临床决策错误.
准确的生化测试结果对于有效的患者管理至关重要.
了解血液溶解的影响对于实验室质量保证至关重要.

研究的目的:

评估不同血解水平对常规生化分析物的影响.
为可靠的样本报告建立局部溶血指数 (HIX) 截止值.
为了确定由于血液溶解而导致的分析物特定干扰模式.

主要方法:

使用30个健康个体血清样本进行实验研究.
使用DIRUI CS-1200分析仪测量模拟血液溶解前后的分析剂度.
模拟血解通过添加自主血解盐,由HIX分级.
与基线的偏差计算和与CLIA的总允许误差 (TEa) 的比较.

主要成果:

所有分析品都表现出正偏差与增加的血液溶解;干扰有显著的差异.
LDH,尿酸,AST和总胆红素显示出与血液溶解相关的干扰最多.
轻度血液溶解 (HIX <0.5) 对任何分析物 (p <0.05) 都没有超过TEa极限.
适度和严重的血解超过了大多数分析物的可接受误差限值.

结论:

血液溶解干扰是取决于分析剂和系统的.
对于报告轻度血解样本,建议血解指数 (HIX) 截止值为0.5.
实验室应在当地验证发现,以提高分析质量并减少样本拒绝.

关键词:

生物化学生物化学血液溶解 (hemolysis) 是一种血液溶解指数血溶性指数干扰干扰是干扰的

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