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流行病学研究和分子表征在埃及牛的块性皮肤疾病在埃及

Heba Hassan El-Nady¹, Ahmed Mansour², Mohamed Ibrahim Eissa¹

¹Department of Animal Medicine, Infectious Diseases, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt, zu.edu.eg.

Veterinary medicine international

|March 2, 2026

概括

在埃及爆发的型皮肤病病毒 (LSDV) 疫情显示出高发病率. 分子分析揭示了EEV糖蛋白基因的遗传变异,使得感染和接种疫苗的动物之间有区别.

科学领域:

兽医病毒学兽医病毒学
流行病学流行病学
分子生物学分子生物学

背景情况:

块性皮肤病病毒 (LSDV) 是一种影响牲畜的显著的天花病毒,其特点是患病率高,死亡率低.
最近在埃及爆发的疫情凸显了对LSDV的流行病学和分子特征的需要.

研究的目的:

为了调查LSDV在埃及最近爆发期间的流行病学.
使用PCR和EEV糖蛋白基因的序列分析对LSDV分离物的分子分析.
评估区分感染动物和接种疫苗的可能性 (DIVA).

主要方法:

从埃及四个省份的10个群体中收集了470头牛的流行病学数据.
传统的聚合酶连锁反应 (PCR) 来放大细胞外包裹病毒 (EEV) 糖蛋白基因.
对EEV糖蛋白基因的序列分析和与疫苗菌株的比较.
免疫胆膜和马丁 - 达比牛细胞系.

主要成果:

病发率为35.53%和死亡率为5.32%被记录下来.
对于LSDV爆发的重要预测因素包括动物年龄,放牧系统,水源和未被隔离的动物的引入.
在95.65%的样本中,PCR证实了LSDV的存在.
序列分析显示与ARRIAH LSD VAC相比,不同的核酸和氨基酸变异,确定了单核酸多态 (SNP).

结论:

该研究阐明了导致埃及LSDV爆发的关键流行病学因素.
EEV糖蛋白基因序列变异为开发DIVA策略提供了基础.
建议包括疫苗接种和用于疾病控制的驱剂.

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