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基于水凝的管上的气道,具有可输注的内皮层层和外向上皮质化.
Ali Doryab1,2, Jack F Murphy1,2, Michael Bartolf-Kopp3
1Department of Engineering, University of Cambridge, Cambridge, UK.
研究人员使用工程水凝和脚手架开发了一种新的气道-管道模型. 这种先进的临床前工具更好地模拟了肺部的复杂性,改善了慢性肺部疾病的药物开发.
科学领域:
- 生物医学工程 生物医学工程
- 肺部医学 肺部医学
- 组织工程是组织工程.
背景情况:
- 慢性肺部疾病在全球造成显著的死亡率,治疗选择有限.
- 临床前模型缺乏复杂性,无法完全复制肺生理,阻碍了药物开发.
- 现有的芯片上的气道模型通常是平面的,并且缺乏用于动态细胞相互作用的可 perfusable 血管组件.
研究的目的:
- 开发一种先进的气道-管道模型,克服当前临床前肺模型的局限性.
- 创建一个平台,将工程外细胞矩阵 (ECM) 与动态培养的管状支架集成在一起.
- 能够更好地回顾肺组织复杂性,以改善药物查和研究.
主要方法:
- 一个管状结构的制造,将工程ECM (EnECM) 水凝与化电写 (MEW) 脚手架相结合.
- 在EnECM水凝中嵌入来自患者的初级人肺微血管内皮细胞,形成可 perfusable 光.
- 在外部表面培养初级人类支气管上皮细胞,以建立空气-液体接口 (ALI).
- 实施脉冲性输液,将营养物质和机械刺激 (剪切应力,循环拉伸) 输送到内皮层.
主要成果:
- EnECM/MEW结构为动态培养提供了机械支持,而不会影响细胞行为.
- 该模型成功地在ALI确立了可输注的内皮膜光和面向外的上皮质.
- 脉冲性 perfusion 维持了 ALI 培养,同时提供了必需的营养素和机械线索.
- 该平台展示了下一代气道在芯片应用程序的多功能性.
结论:
- 一个基于水凝的新型气道-管道平台成功建立.
- 这种模型通过更好地模仿肺组织复杂性和血管相互作用来增强临床前肺部研究.
- 该平台为推进慢性肺部疾病的精密治疗开发提供了新的机会.
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