Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

4.4K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
4.4K
Integration of Synaptic Events01:28

Integration of Synaptic Events

5.0K
Synaptic integration mainly includes the summation of graded potentials. Graded potentials, regardless of their type, cause subtle alterations in membrane voltage, resulting in either depolarization or hyperpolarization. These incremental changes, when combined or summed, can propel the neuron toward its threshold. Consider, for example, a membrane experiencing a +15 mV shift, causing it to depolarize from -70 mV to -55 mV. In this scenario, graded potentials govern the membrane's ability to...
5.0K
GPCR Desensitization01:12

GPCR Desensitization

8.4K
G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
8.4K
G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

6.3K
GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory...
6.3K
Chemical Synapses01:26

Chemical Synapses

12.1K
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
12.1K
Chemical Synapses01:26

Chemical Synapses

5.2K
Chemical synapses are specialized sites between two neurons or between a neuron and a non-neuronal cell like a muscle, glandular or sensory cell.
Because chemical synapses depend on the release of neurotransmitter molecules from synaptic vesicles to pass on their signal, there is an approximately one millisecond delay between when the axon potential reaches the presynaptic terminal and when the neurotransmitter leads to opening of postsynaptic ion channels. Additionally, this signaling is...
5.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Acute changes in ankle dorsiflexor strength and fNIRS-Derived cortical activation following a single session of neuromuscular electrical stimulation in healthy older adults.

Frontiers in aging·2026
Same author

Corrigendum to "Gut microbiota metabolite Urolithin B inhibits chondrocyte ferroptosis by rewriting iron homeostasis via FGFR3/NCOA4/FTH1 axis, alleviating osteoarthritis" [Phytomedicine (Volume 148, 25 November 2025, 157292)].

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same author

Optical cooling by interfacial charge transfer in 2D heterostructures.

Nature·2026
Same author

Transferable Two-Dimensional Hydrophobic Metal-Organic Framework Encapsulation of MoS<sub>2</sub>-Based Humidity-Resistant NO<sub>2</sub> Sensors.

ACS sensors·2026
Same author

Fluorogenic probes for intracellular copper detection and the study of cuproptosis.

Methods in cell biology·2026
Same author

Two-dimensional melt growth of large-scale, single-crystalline hybrid organic-inorganic perovskite films.

Nature communications·2026
Same journal

Canonical and phosphoribosyl ubiquitination coordinate to stabilize a proteinaceous structure surrounding the <i>Legionella</i>-containing vacuole.

eLife·2026
Same journal

Celldetective, an AI-enhanced image analysis tool for unraveling dynamic cell interactions.

eLife·2026
Same journal

Dynamic assembly of malate dehydrogenase-citrate synthase multienzyme complex in the mitochondria.

eLife·2026
Same journal

Autosomal allelic inactivation at loci with variable replication timing and dosage sensitivity.

eLife·2026
Same journal

Cribriform plate microenvironment assembles a suppressive myeloid network during EAE-induced neuroinflammation.

eLife·2026
Same journal

Proteomic composition and mutual assembly of the C2a projection in vertebrate motile cilia.

eLife·2026
查看所有相关文章

相关实验视频

Updated: Mar 3, 2026

Identification and Classification of Position-specific GABAA Receptor Subunit Missense Variants for Their Role In Hippocampal Pyramidal Neurons
08:04

Identification and Classification of Position-specific GABAA Receptor Subunit Missense Variants for Their Role In Hippocampal Pyramidal Neurons

Published on: June 6, 2025

1.6K

使用子突触向的GCaMP8变体来解决突触事件.

Jiawen Chen1,2, Junhao Lin1, Kaikai He1,2

  • 1University of Southern California, Department of Neurobiology, Los Angeles, United States.

eLife
|March 2, 2026
PubMed
概括
此摘要是机器生成的。

下一代遗传编码指标 (GCaMP8) 和CaFire分析工具显著提高了可视化神经活动的速度和灵敏度.

关键词:
D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. D. melanogaster. melanogaster. D. melanogaster. melanogaster. D. melanogaster. D. melanogaster. melanogaster. D.和是最重要的.在基因编码的基因编码.这是一个指标指标指标.神经肌肉结节是一个神经肌肉结节.神经科学 神经科学这是一个比率计的比率计.突触终端中的突触终端.

更多相关视频

Evaluation of Synaptic Multiplicity Using Whole-cell Patch-clamp Electrophysiology
10:52

Evaluation of Synaptic Multiplicity Using Whole-cell Patch-clamp Electrophysiology

Published on: April 23, 2019

13.8K
Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals
12:01

Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals

Published on: October 1, 2014

9.2K

相关实验视频

Last Updated: Mar 3, 2026

Identification and Classification of Position-specific GABAA Receptor Subunit Missense Variants for Their Role In Hippocampal Pyramidal Neurons
08:04

Identification and Classification of Position-specific GABAA Receptor Subunit Missense Variants for Their Role In Hippocampal Pyramidal Neurons

Published on: June 6, 2025

1.6K
Evaluation of Synaptic Multiplicity Using Whole-cell Patch-clamp Electrophysiology
10:52

Evaluation of Synaptic Multiplicity Using Whole-cell Patch-clamp Electrophysiology

Published on: April 23, 2019

13.8K
Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals
12:01

Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals

Published on: October 1, 2014

9.2K

科学领域:

  • 神经科学是一个神经科学.
  • 分子生物学分子生物学
  • 遗传学 遗传学 是一个

背景情况:

  • 基因编码的指标 (GCaMP) 对于可视化神经活动至关重要.
  • 以前的GCaMP版本和合成染料在速度和灵敏度方面存在限制,特别是在突触区.
  • 电生理学是一个黄金标准,但缺乏空间分辨率.

研究的目的:

  • 为突触区设计和验证下一代基于GCaMP8的指标.
  • 开发一种用于量化信号的自动化分析程序 (CaFire).
  • 与现有方法相比,证明GCaMP8传感器的性能提高.

主要方法:

  • 工程GCaMP8指标针对Drosophila的前突触,活跃区域和后突触区.
  • 对传感器性能与以前的GCaMP版本和合成染料的验证.
  • 开发基于Python的CaFire程序,用于自动化信号量化.
  • 应用CaFire来分析突触前,活性区和突触后动态.

主要成果:

  • 与以前的版本和合成染料相比,工程设计的GCaMP8传感器表现出卓越的性能.
  • 该CaFire程序实现了唤起和自发信号的自动量化.
  • 一个预突触GCaMP8m传感器准确地捕获了具有高灵敏度的生理学相关变化.
  • 一个针对活跃区域的传感器区分了释放地点之间的差异.
  • 一个后突触GCaMP8m传感器检测到量子事件的分辨率与电生理学相比较.

结论:

  • 下一代GCaMP8传感器,当针对突触区时,实现速度和灵敏度与化学染料和电生理学相竞争.
  • 该CaFire分析工具有助于对突触动态的可靠量化.
  • 这些进步使得能够对突触中的信号进行高分辨率的研究.