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长ipeptin生物合成基因集群的异质表达和体内特征

Yuwei Duan¹, Menghan Shi¹, Weifeng Sun¹

¹State Key Laboratory of Microbial Technology, Shandong University, Qingdao 266237, China. zhonggn@sdu.edu.cn.

Organic & biomolecular chemistry

|March 2, 2026

概括

研究人员阐明了长ipeptin A,一个拉索的生物合成. 他们确定了关键酶,包括细胞染色体P450和DUF6919蛋白,揭示了基修饰和RiPPs中硫安装的新机制.

科学领域:

生物化学生化学
分子生物学分子生物学
自然产品生物合成自然产品生物合成

背景情况:

拉索是通过核糖体合成和翻译后修改的 (RiPPs),具有独特的线状环结构.
来自*Longimycelium tulufanense*的Longipeptin A,具有一个Trp-Trp C-N交叉链,5-基和一个三价硫S-甲基甲.
与*L. tulufanense*有关的遗传挑战,之前对长ipeptin生物合成的研究是有限的.

研究的目的:

为了异质地表达longipeptin生物合成基因集群并定义其基本基因.
描述细胞染色体P450 (LopF/LopG) 和DUF6919蛋白 (LopH) 在longipeptin生物合成中的作用和时间.
阐明RiPPs中的Trp-Trp交联,基化和S-甲基化机制.

主要方法:

长ipeptin生物合成基因集群的异质表达.
鉴定长ipeptin生物合成的必要基因.
描述LopF,LopG和LopH的酶活性.

主要成果:

定义了longipeptin生物合成基因集群的边界和基本基因.
LopF催化了Trp-Trp C-N交联,LopG调解了托化,LopH作为S-甲基化的一种新型甲基转移酶.
所有的修改都发生在巨乳化后,交叉链接是甲基化的先决条件.

结论:

建立了一个研究P450催化拉索交联的框架.
首次实现了DUF6919家族蛋白质 (LopH) 的功能性表征.
揭示了RiPP中硫安装的不同策略.

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