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来自Coptotermes formosanus的内葡萄糖酶的发现和功能性表征,通过酵母表面显示显示通过增强的纤维素水解

Jingfang Sun¹, Chang Cao¹, Yushi Chen¹

¹School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, China.

Applied and environmental microbiology

|March 2, 2026

概括

研究人员发现了来自白的八种新型内葡萄糖酶,CTEG6在分解纤维素方面表现出高效率. 这一发现提供了一种可持续的方法,用于将农业废物转化为有价值的化学品.

科学领域:

生物技术和生物工程生物技术和生物工程
酶学是一种酶学.
微生物生物技术微生物生物技术

背景情况:

微生物纤维素降解是农业和林业残留物可持续转化的关键.
需要新的酶来提高生物质转化效率.

研究的目的:

发现和描述用于纤维素降解的新型内葡萄糖酶.
确定最有效的酶并了解其优化生物质转化机制的机制.

主要方法:

选一个白cDNA库的内葡萄糖酶活动.
在Saccharomyces cerevisiae中的异质表达和酶表征.
定量PCR (qPCR) 用于基因拷贝数分析.
结构建模 (AlphaFold2) 和分子对接以阐明机制.
局部定向突变发生和体外/体内活性测试.
为纤维素水解优化发酵过程.

主要成果:

发现并表达了8种新的内葡萄糖酶 (CTEG1,CTEG3-CTEG9).
CTEG6表现出最高的水解活性 (≈30 U/mg) 和全细胞性能 (1.51 mg/mL降解糖).
基因拷贝数的变化影响了酶表达水平.
关键残留物 (A11,D251,S258) 与基质结合和催化有关.
这些残留物的突变发生显著降低了酶活性.
在最佳条件下,CTEG6有效地化了各种形式的纤维素 (CMC-Na,MCC,天然纤维素).

结论:

CTEG6是一种非常有前途的生物催化剂,用于高效的纤维素水解.
对基质结合和催化物的机理洞察力为酶优化提供了基础.
这项工作有助于开发可再生能源和绿色制造的可持续生物转换技术.

关键词:

科普特语中的术语是formosanus.这种植物是Saccharomyces cerevisiae.纤维素的水解是纤维素的水解.结核糖酸酶的使用方法酵母表面显示器显示酵母表面.

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