纤维细胞生长因子2对来自小鼠四肢和眼外肌肉的肌肉前体细胞的影响
Austin J Winker1,2, Laura L Johnson1, Ria Jadhav1
1Department of Ophthalmology and Visual Neurosciences, University of Minnesota, Minneapolis, Minnesota, United States of America.
PloS one
|March 2, 2026
概括
纤维细胞生长因子2 (FGF2) 促进了骨肌肉前体细胞的增殖,但抑制了它们的融合到髓管中. 这项研究揭示了FGF2在肌肉发育中的复杂作用以及对阿珀特综合征等疾病的潜在影响.
科学领域:
- 肌肉生物学 肌肉生物学
- 细胞和分子医学 细胞和分子医学
- 发展生物学 发展生物学
背景情况:
- 纤维细胞生长因子2 (FGF2) 已被公认为其参与骨肌肉的发育和生长.
- 肌源性前体细胞对于肌肉再生和发育至关重要.
- 了解FGF2对不同肌性细胞种群的特定影响至关重要.
研究的目的:
- 研究FGF2在眼外肌肉和四肢骨肌肉中的两个不同的肌源性前体细胞群 (EECD34和PAX7阳性细胞) 上的体外效应.
- 确定FGF2如何影响这些细胞的增殖和分化.
- 阐明FGF2影响肌管形成和肌纤维发育的机制.
主要方法:
- 通过光激活细胞分类 (FACS) 从小鼠前和外眼肌中分离了EECD34和PAX7阳性的肌源性前体细胞.
- 在有或没有FGF的增殖或分化介质中培养孤立细胞2.
- 测试用于测量细胞增殖,分化 (desmin表达) 和融合到髓管 (myomerger表达).
主要成果:
- FGF2显著增加了来自两种肌肉类型的EECD34和PAX7阳性细胞的增殖.
- 治疗FGF2导致了desmin阳性单核细胞的百分比增加,但减少了融合到多核髓管.
- 免疫染显示,在FGF2的存在下,肌肉聚合物表达显著减少,这表明融合受损.
结论:
- 在实验室中,FGF2对骨肌肉表现出性作用,促进增殖,同时抑制合成多核肌纤维的融合.
- 观察到的神经聚合体表达的减少提供了减少神经纤维大小和数量的潜在机制.
- 这些发现可能解释与阿珀特综合征相关的眼外肌肉中肌纤维异常,这是由于FGF受体2突变引起的.
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