Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

What is Genetic Engineering?00:49

What is Genetic Engineering?

80.7K
Overview
80.7K
The Central Dogma01:20

The Central Dogma

34.5K
The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
34.5K
Synthetic Biology02:55

Synthetic Biology

5.7K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
5.7K
Reporter Genes02:11

Reporter Genes

13.5K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
13.5K
Genome Size and the Evolution of New Genes03:21

Genome Size and the Evolution of New Genes

9.3K
While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.
9.3K
Transgenic Organisms00:53

Transgenic Organisms

34.1K
Overview
34.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Combined biosynthesis and site-specific incorporation of phenylalanine derivatives from aryl aldehydes or carboxylic acids.

Nature communications·2026
Same author

Glutamate facilitates root colonization by plant growth-promoting rhizobacteria <i>Bacillus subtilis</i> in tomato seedlings.

Microbiology spectrum·2026
Same author

Development of a flow cytometric method to evaluate the impact of N-terminal sequences on protein stability.

Methods in enzymology·2025
Same author

Combinatorial mutagenesis of N-terminal sequences reveals unexpected and expanded stability determinants of the <i>Escherichia coli</i> N-degron pathway.

bioRxiv : the preprint server for biology·2025
Same author

Engineered orthogonal and obligate bacterial commensalism mediated by a non-standard amino acid.

Nature microbiology·2025
Same author

Design of an exclusive obligate symbiosis for organism-based biological containment.

bioRxiv : the preprint server for biology·2025

相关实验视频

Updated: Mar 4, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

9.1K

通过代谢工程推进活细胞中的遗传密码扩展.

D'Jana R Wyllis1, Saloni P Gupta1, Shelby R Anderson1

  • 1Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, Delaware, USA;

Annual review of chemical and biomolecular engineering
|March 2, 2026
PubMed
概括

遗传密码扩展 (GCE) 能够创建具有新型构建块的蛋白质,即非标准氨基酸 (nsAAs). nsAAs的生物合成克服了化学合成的局限性,推进了蛋白质工程和合成生物学.

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 合成生物学 合成生物学

背景情况:

  • 遗传密码扩展 (GCE) 允许将超出20个标准的非标准氨基酸 (nsAAs) 纳入蛋白质中.
  • 这扩展了蛋白质化学功能,用于各种研究和应用.
  • 目前的GCE方法通常需要化学合成的nsAAs,这构成了采购挑战.

研究的目的:

  • 审查NSAA生物合成工程系统的进展.
  • 探索代谢工程与GCE的整合,以提高蛋白质的生产.
  • 为GCE和nsAA生物合成领域的创新提供视角.

主要方法:

  • 关于NSAA生产的遗传密码扩展和代谢工程的文献综述.
  • 对工程系统进行分析,以在体内进行nsAAs的生物合成.
  • 讨论合成生物学应用的综合方法.

主要成果:

  • 工程系统现在可以在宿主细胞内生物合成各种nsAAs.
  • 将代谢工程与GCE相结合,为化学 nsAA采购提供了一个可持续的替代方案.
  • 从这些综合方法中,新的化学目标和合成生物学应用正在出现.

更多相关视频

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

9.6K
Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression
09:08

Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression

Published on: November 4, 2025

414

相关实验视频

Last Updated: Mar 4, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

9.1K
Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials
10:28

Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials

Published on: March 9, 2017

9.6K
Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression
09:08

Rapid Optimization of a Light-Inducible System to Control Mammalian Gene Expression

Published on: November 4, 2025

414

结论:

  • nsAA生物合成代表了一项重要的创新,克服了GCE的局限性.
  • 代谢工程和GCE的整合对于未来的蛋白质工程至关重要.
  • 这一领域对推进基础研究和应用生物技术具有很大的前景.