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细胞原蛋白抗原测试结果受到狗的尿液采集方法的影响

Katie Como¹, Kathleen M Aicher², Song Zhang³

¹Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX.

American journal of veterinary research

|March 2, 2026

概括

标准化尿液采集,以诊断犬类血清质症至关重要. 无菌囊细胞切割提供了最低的错误阳性率,提高了质体尿抗原酶免疫检测结果的诊断准确度.

科学领域:

兽医诊断兽医诊断兽医诊断兽医诊断
菌类学菌类学是指菌类学.
狗的传染病狗的传染病

背景情况:

血清尿抗原酶免疫试验 (EIA) 对于诊断狗的血清质症至关重要.
缺乏标准化的尿液采集方法可能会影响诊断的准确性.
评估不同的收集技术是必要的,以优化EIA的表现.

研究的目的:

评估三种尿液采集方法之间的一致性:自由捕获 (FC),囊 (cysto) 和受污染的囊 (contam).
使用MV抗原EIA,确定与每个采集技术相关的错误阳性率 (FPRs).
提供以证据为基础的建议,以优化尿样采集,用于诊断犬类组织等离子体病.

主要方法:

一项前性研究涉及30只可疑患有组织等离子体病的狗和30只健康的对照.
在24小时内从每只狗身上采集三个尿样 (FC,cysto,contam).
使用McNemar测试和Cohen κ系数进行统计分析以评估一致性;临床诊断作为PFR计算的参考标准.

主要成果:

在囊和接触样本 (κ = 0.68) 之间观察到实质性的一致性,在囊和交流样本 (κ = 0.45) 和交流样本和接触样本 (κ = 0.42) 之间观察到适度的一致性.
总的假阳性率为16%.
囊样本表现出最低的FPR (4%),其次是自由捕获 (7%) 和受污染的囊 (11%).

结论:

所有评估的尿液采集技术都显示出对Histoplasma尿液抗原EIA的合理一致.
自由捕获和受污染的囊泡切割样本更容易产生错误阳性.
临床医生应考虑使用无菌囊细胞检测或确认其他方法的模两可的结果,以提高犬类组织等离子体病的诊断准确度.

关键词:

血清质体中的血清体.协议协议协议协议协议抗原是一种抗原.狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗狗尿液尿液尿液

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