循环节律障碍通过干扰卵巢类固醇激素合成途径诱导PCOS类现象
ChengYu Feng1, LiYing Guo1, Xin Gao1
1School of life science and technology, Inner Mongolia University of Science & Technology, Baotou 014010, China.
概括
慢性压力,特别是昼夜节律障碍,通过损害生殖内分泌系统,导致雌性小鼠的不孕. 这种压力模型模仿了多囊卵巢综合征 (PCOS),并揭示了新的治疗点.
科学领域:
- 生殖内分泌学 生殖内分泌学
- 压力生理学 压力生理学
- 基因组学就是基因组学.
背景情况:
- 生殖功能障碍通常与慢性压力有关.
- 了解压力诱导不孕症的内分泌机制至关重要.
研究的目的:
- 为了调查慢性压力是否会导致雌性小鼠的不孕.
- 阐明女性生殖内分泌系统的破坏性机制.
- 确定与压力相关的生殖功能障碍的潜在治疗点.
主要方法:
- 在雌性ICR小鼠中建立了三种慢性压力模型:慢性克制压力 (CRS),昼夜节律扰乱 (CR) 和慢性不可预测的轻度压力 (CUMS).
- 评估HPA轴激活,生殖器官系数,雌性周期, litter 大小和荷尔蒙水平 (皮质,上腺素,LH,孕激素,雌激素,黑激素,丸激素).
- 进行了卵巢转录组分析以确定差异表达的基因,并进行了功能丰富分析.
主要成果:
- 循环节律扰乱 (CR) 最显著地激活了HPA轴.
- 治疗CR降低了体重,卵巢/子宫系数,破坏了雌性周期,增加了动脉毛囊,减少了 litter 尺寸.
- 激素测试显示LH,孕激素,雌激素和黑激素降低,丸激素升高,类似于多囊性卵巢综合征 (PCOS) 形状. 关键的雌激素合成基因被下调,而LHR显示补偿性上调.
结论:
- 循环节律障碍通过降低雌激素合成途径基因的调节,损害生殖功能.
- 实验诱导的压力可以导致PCOS类型的表型.
- 这项研究提供了新的机制性见解,并确定了与压力相关的PCOS的潜在治疗点.
相关概念视频
02:19Circadian Rhythms and Gene Regulation
4.6K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.6K
01:30Hormonal Control of the Ovarian Cycle
7.2K
The ovarian cycle is meticulously regulated by the hypothalamic-pituitary-gonadal axis. This cycle orchestrates the release of a mature oocyte, essential for reproduction.
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
Before puberty, the hypothalamus releases GnRH in a low frequency, low amplitude pulsatile manner. This along with the immature hypothalamic-pituitary-gonadal axis activity, results in low estrogen levels and the absence of a fully functional ovarian cycle. At puberty, GnRH secretion increases in both frequency and...
7.2K
01:22Hormonal Regulation of the Menstrual Cycle
1.9K
The ovarian cycle regulates endometrial changes throughout a single menstrual cycle via the coordinated action of gonadotrophin-releasing hormone (GnRH) and gonadotrophins.
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
At puberty, GnRH begins a pulsatile release pattern, which triggers the anterior pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). The frequency and amplitude of GnRH pulses vary across the menstrual cycle, with faster pulses favoring LH release and slower pulses favoring FSH...
1.9K
01:27Ovarian Cycle
4.8K
The menstrual cycle includes a critical component known as the ovarian cycle, which undergoes two main phases each month—the follicular phase and the luteal phase. The follicular phase is variable and averaging around 14 days. Ovulation, triggered by a surge in luteinizing hormone (LH), marks the transition between the two phases. The second phase, the luteal phase, is relatively consistent, lasting approximately 14 days, and is marked by the activity of the corpus luteum. While a cycle...
4.8K
02:07Oogenesis
70.7K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
70.7K
01:24Disorders of the Female Reproductive System
4.7K
The female reproductive system can be affected by several disorders, including Premenstrual Syndrome (PMS), Premenstrual Dysphoric Disorder (PMDD), endometriosis, and various forms of cancer. PMS and PMDD are cyclical conditions that cause physical and emotional distress, with symptoms that include edema, mood swings, and food cravings. PMDD is a more severe form of PMS characterized by increased symptom severity that peaks during the luteal phase and tends to improve or resolve shortly after...
4.7K