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相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...

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相关实验视频

Updated: Jun 6, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

使用TMT-Integrator和FragPipe计算平台对同位素定量蛋白质组数据进行分析.

Hui-Yin Chang1,2, Yamei Deng1, Ruohong Li1

  • 1Department of Pathology, University of Michigan, Ann Arbor, MI, USA.

Nature communications
|March 2, 2026
PubMed
概括
此摘要是机器生成的。

TMT-Integrator是一种新的生物信息学工具,通过提供来自相对和绝对量化 (iTRAQ) 实验的双重质量标签 (TMT) 和同位体标签的综合报告来增强定量蛋白质组学. 与现有方法相比,它提供了更强大的蛋白质和酸量化方法.

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TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
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TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

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Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

相关实验视频

Last Updated: Jun 6, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis
07:44

TMT Sample Preparation for Proteomics Facility Submission and Subsequent Data Analysis

Published on: June 8, 2020

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 生物信息学是一种生物信息学.
  • 质谱测量质量谱测量

背景情况:

  • 像TMT和iTRAQ这样的同质质标签对于多重量的定量蛋白质组学至关重要.
  • 准确的和蛋白质量化对于生物发现至关重要.

研究的目的:

  • 推出TMT-Integrator,这是一个生物信息学工具,用于处理TMT和iTRAQ量化结果.
  • 在多个生物层面 (基因,蛋白质,,PTM站点) 提供综合性报告.
  • 在FragPipe计算平台中评估TMT-Integrator的性能.

主要方法:

  • 在FragPipe计算平台中开发和集成TMT-Integrator.
  • 分析了五个不同的TMT数据集,包括ccRCC,大肠杆菌入和人类细胞溶解物.
  • 使用OmicsEV.使用MaxQuant和Proteome Discoverer进行性能比较.

主要成果:

  • TMT-Integrator通过FragPipe量化了大肠杆菌和ccRCC全蛋白质组数据集中的更多蛋白质.
  • 在ccRCC蛋白质组数据集中量化了更多的化部位.
  • 与TMT-Integrator相结合的FragPipe显示出更优越,更强大的量化性能.

结论:

  • TMT-Integrator是一个多功能工具,用于全面分析TMT和iTRAQ定量蛋白质组学数据.
  • 与FragPipe的集成为定量蛋白质组学提供了一个强大而稳健的工作流.
  • 这种工具有助于分析复杂的蛋白质组数据集,包括蛋白质组学.