单细胞转录学揭示了严重的SARS-CoV-2相关的儿科脑病变中热冲击蛋白失调
Takako Suzuki1, Yoshitaka Sato2, Motomasa Suzuki3
1Department of Pediatrics, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan. suzuki.takako.j9@f.mail.nagoya-u.ac.jp.
Scientific reports
|March 2, 2026
概括
在儿童中,与SARS-CoV-2相关的严重急性脑病变显示出升高的热冲击蛋白HSPA1A和HSPB1.1. 这些应激反应蛋白可能作为儿童COVID-19中严重神经复杂症的生物标志物.
科学领域:
- 免疫学 免疫学 免疫学
- 神经科学是一个神经科学.
- 病毒学 病毒学
背景情况:
- 与SARS-CoV-2相关的儿童严重急性脑病变/脑炎 (AE),特别是Omicron变种,越来越多地报告.
- 像急性暴发性脑 (AFCE) 和出血性休克脑内病综合征 (HSES) 这样的疾病在儿科COVID-19患者中存在显著的发病率和死亡率.
- 导致SARS-CoV-2相关AE的精确致病机制在很大程度上是未知的.
研究的目的:
- 在儿科患者中调查SARS-CoV-2相关的严重AE的免疫和分子机制.
- 为了确定儿童COVID-19中严重的神经并发症的潜在生物标志物.
主要方法:
- 由SARS-CoV-2引起的严重AE (AFCE/HSES) 的儿科患者的外周血液单核细胞的单细胞RNA测序.
- 与轻度AE患者,发烧性和儿童COVID-19数据集的数据进行比较分析,而无神经参与.
- 细胞与细胞通信分析和酶相关免疫吸收试验 (ELISA) 以验证基因表达发现.
主要成果:
- 在严重AE的急性阶段观察到活化B细胞种群的显著扩张.
- 鉴定了巨细胞迁移抑制因子信号,但不特定于SARS-CoV-2相关的AE.
- 热冲击蛋白基因HSPA1A和HSPB1在严重AE患者的免疫细胞中被选择性上调,血/血清蛋白水平升高通过ELISA证实.
结论:
- 免疫细胞中HSPA1A和HSPB1的升级是儿童严重的SARS-CoV-2相关AE的独特特征.
- HSPA1A和HSPB1显示出在儿童COVID-19中严重的神经并发症的生物标志物的潜力.
- 应激反应途径可能在严重的SARS-CoV-2-相关AE的发病过程中发挥重要作用.
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