Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

8.5K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
8.5K
Homologous Recombination02:31

Homologous Recombination

58.8K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
58.8K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

5.1K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.1K
Assembly of Cytoskeletal Filaments01:18

Assembly of Cytoskeletal Filaments

17.4K
Cytoskeletal filaments are polymeric forms of smaller protein subunits. However, individual cytoskeletal filaments may easily disassemble or associate with other similar filaments to form rigid structures. Microfilaments, made of actin monomers, rely on actin-binding proteins to form bundles and create networks of individual actin filaments. Microtubules rely on microtubule-associated proteins (MAPs) to form sturdy cylindrical structures. However, the proteins involved in forming complex...
17.4K
Mechanism of Filopodia Formation01:39

Mechanism of Filopodia Formation

2.5K
Filopodia are thin, actin-rich cellular protrusions that play an important role in many fundamental cellular functions. They vary in their occurrence, length, and positioning in different cell types, suggesting their diverse roles.
Their main function is to guide migrating cells during normal tissue morphogenesis or cancer metastasis by recognizing and making initial contacts with the extracellular matrix. However, they can also act as stationary cell anchors or help to establish communication...
2.5K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

2.4K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
2.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Occurrence of Human Norovirus and Hepatitis A Virus in Domestic Agricultural Waters.

Food safety (Tokyo, Japan)·2026
Same author

Architecture and function of holocentric CENP-A-independent inner kinetochores.

Science advances·2026
Same author

NAT10 deficiency in aging drives impaired liver regeneration by disrupting PARP10 in an ac4C-dependent manner.

Clinical and molecular hepatology·2026
Same author

Optimization Leading to a Potent and Selective Cbl‑b Inactive-State Inhibitor That Demonstrated <i>In Vivo</i> Efficacy.

ACS medicinal chemistry letters·2026
Same author

Optimization and <i>In Vivo</i> Characterization of a Series of Cbl‑b Inactive-State Inhibitors.

ACS medicinal chemistry letters·2026
Same author

Antibody-Mediated Delivery of BRM/BRG1 Protein Degraders Affords Strong Antitumor Efficacy in Multiple BRM-Dependent Non-Small Cell Lung Cancer Xenograft Models.

Journal of medicinal chemistry·2026
Same journal

Daily briefing: How cooperation built the world.

Nature·2026
Same journal

Deep-sea oddities and boatloads of other new species - June's best science images.

Nature·2026
Same journal

From cloning to gene-editing: the enduring legacy of Dolly the sheep.

Nature·2026
Same journal

Time to give hydration breaks the red card? What science says about keeping cool.

Nature·2026
Same journal

Universities are relying on AI-detection software to catch cheating. How well do the programs work?

Nature·2026
Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026
查看所有相关文章

相关实验视频

Updated: May 5, 2026

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

13.8K

BCDX2-CX3和DX2-CX3复合体组装并稳定RAD51的纤维.

Christopher W Koo1, Jiaqi Xiao1, Sebastien Coassolo2

  • 1Protein Sciences, Genentech Inc., South San Francisco, CA, USA.

Nature
|March 2, 2026
PubMed
概括
此摘要是机器生成的。

在DNA修复和预防癌症方面至关重要的5个RAD51对应物形成了一个超级复合体. 这个复合体作为DNA修复"加载器"和"",澄清了它们在基因组完整性中的作用.

更多相关视频

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

9.0K
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.1K

相关实验视频

Last Updated: May 5, 2026

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

13.8K
Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

9.0K
Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.1K

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物化学 生物化学

背景情况:

  • 同源重组 (HR) 修复DNA双链断裂,保持基因组的完整性.
  • RAD51对应物 (RAD51B,RAD51C,RAD51D,XRCC2,XRCC3) 调节RAD51重组酶组合,这对HR至关重要.
  • HR和RAD51对象的失调与癌症和遗传疾病有关.

研究的目的:

  • 为了阐明在DNA修复中五个人类RAD51对应物的组合和功能.
  • 确定RAD51与单链DNA (ssDNA) 结合的对应组合体的结构结构.
  • 定义RAD51对应复合体在同源重组途径中的不同作用.

主要方法:

  • 生物化学试验用于研究复杂的形成和ATPase活性.
  • 单链DNA (ssDNA) 的结合研究.
  • 对RAD51模拟组件的结构分析.

主要成果:

  • 所有五个RAD51对应物组装成一个单一的ATP依赖的超复合体 (BCDX2-CX3-RAD51) 在ssDNA上.
  • 一个RAD51B独立的DX2-CX3复合体 (RAD51D-XRCC2-RAD51C-XRCC3) 作为一个稳定的RAD51.
  • 确定了不同的功能模块,一个动态的"加载器" (BCDX2-CX3) 和一个稳定的"" (DX2-CX3).

结论:

  • 提出了HR中人类RAD51对应函数的统一机制.
  • 已识别的超复杂架构为RAD51光纤形成提供了一个模板.
  • 这项研究提供了一个原子框架,以了解RAD51对应物中与癌症相关的突变.