相关实验视频
Updated: Jun 20, 2026

11:09
An Easy Method for Plant Polysome Profiling
Published on: August 28, 2016
描述miRNA循环环境及其在Arabidopsis中的调节
Yixiao Fang1, Ning Li2, Youhong Fan1
1State Key Laboratory of Genetics and Development of Complex Phenotypes, Zhangjiang mRNA Innovation and Translation Center, School of Life Sciences, Fudan University, Shanghai 200438, China.
The Plant cell
|March 3, 2026
概括
植物微RNA (miRNA) 的稳定性是动态调节的. 这项研究表明,ARGONAUTE 1 (AGO1) 和HUA ENHANCER 1 (HEN1) 保持了miRNA的稳定性,而HWAIIAN SKIRT (HWS) 则调解了特定短寿命miRNA的降解.
科学领域:
- 植物分子生物学 植物分子生物学
- RNA生物学的RNA生物学
- 遗传学 是一个遗传学.
背景情况:
- 微RNA (miRNA) 的积累受到转录,处理和降解的严格调节.
- 控制miRNA循环和活性降解的机制尚未完全理解.
- 研究miRNA稳定性对于理解植物中的基因调节至关重要.
研究的目的:
- 为了在全球范围内划分Arabidopsis thaliana中小RNA循环的景观.
- 确定调节miRNA稳定性和降解的关键因素和机制.
- 了解miRNA动态在植物应激反应中的作用.
主要方法:
- 利用转录抑制与氧化小RNA测序 (OX-sRNA-seq) 结合,捕获2'-O-甲基化小RNA.
- 使用脉冲追踪方法与代谢RNA标记来验证周转率.
- 对AGO1,HEN1和HWS等关键调节因子进行逆基因分析.
主要成果:
- 大多数microRNAs (miRNAs) 和小干扰RNAs (siRNAs) 是稳定的,但miRNAs的一个子集表现出快速的周转.
- miRNAs,miRNAs的补充链,通常寿命短.
- 阿格诺1 (AGO1) 和增强剂1 (HEN1) 对于维持miRNA稳定性至关重要.
- 夏威夷子 (HWS) 对于短命小RNA的降解至关重要,并在氧化应激和酸盐平衡中发挥作用.
结论:
- 植物miRNA稳定性通过不同的降解途径被积极控制.
- HWS是miRNA循环的关键调节者,特别是在短命miRNA中,影响压力反应和营养稳定.
- 这项研究为管理植物miRNA动态的分子机制提供了新的见解.
相关概念视频
Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Riboswitches
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

