Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.8K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Polymyxin B-induced gastric spasm:a case report.

Frontiers in pediatrics·2026
Same author

Suggestive associations between genetically predicted gut microbiota and endometriosis: a two-sample Mendelian randomization study.

Journal of medical microbiology·2026
Same author

Chinese couples' lived experiences and decision-making on pregnancy continuation following prenatal anomaly detection: phenomenological and theory of planned behaviour insights.

BMC pregnancy and childbirth·2026
Same author

NOSIP promotes cell proliferation and motility by targeting SPTAN1 for ubiquitination and degradation in clear cell renal cell carcinoma.

Scientific reports·2026
Same author

Evidence for the Formation of Metabolons Surrounding the Pyruvate Node in the Supercritical CO<sub>2</sub>‑Tolerant <i>Priestia megaterium</i> SR7.

ACS omega·2026
Same author

Beyond ACE2: unveiling the receptor-mediated endocytic dynamic mechanism of SARS-CoV-2 at the single-particle level.

Journal of materials chemistry. B·2026

相关实验视频

Updated: Mar 18, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
08:56

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

7.9K

针对多种生物矩阵的高覆盖量化脂质组分析,使用超高性能液体染色学和并联质谱法.

Qinsheng Chen1, Chenhan Zhang1, Xiaoli Zhang1

  • 1State Key Laboratory of Genetics and Development of Complex Phenotypes, School of Life Sciences, Human Phenome Institute, Zhangjiang Fudan International Innovation Center, Metabonomics and Systems Biology Laboratory at Shanghai International Centre for Molecular Phenomics, Zhongshan Hospital, Fudan University, Shanghai 200032, China.

Analytical chemistry
|March 3, 2026
PubMed
概括

这项研究引入了一种使用UHPLC-MS/MS进行全面脂质分析的新定量脂质学方法. 这种先进的技术改善了酸性脂质的分离,并准确地量化了各种生物样本中的数千种脂质.

更多相关视频

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.8K
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

23.6K

相关实验视频

Last Updated: Mar 18, 2026

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry
08:56

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Published on: March 8, 2020

7.9K
Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis
10:23

Lipid Droplet Isolation for Quantitative Mass Spectrometry Analysis

Published on: April 17, 2017

10.8K
Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
11:00

Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS

Published on: May 20, 2013

23.6K

科学领域:

  • 生物化学 生物化学
  • 分析化学 分析化学
  • 代谢学 代谢学 代谢学

背景情况:

  • 脂粒组的复杂性对量化提出了挑战.
  • 有限的标准和差的染色学阻碍了酸性脂质的分析.
  • 高覆盖度的脂管学对于理解脂质生物功能至关重要.

研究的目的:

  • 开发一种可靠的,高覆盖率的定量脂管学方法.
  • 改善所有脂类,特别是酸性脂类的染色分离.
  • 为脂类子类建立准确的保留时间预测模型.

主要方法:

  • 使用了超高性能液态色谱和双重质谱 (UHPLC-MS/MS).
  • 采用pH和梯度用于增强液态染色学 (LC) 分离.
  • 开发了使用267个脂质标准的定量结构-保留关系模型.
  • 应用多重反应监测 (MRM) 模式用于量化.

主要成果:

  • 对所有脂质,特别是酸性脂质,实现了更好的LC分离.
  • 建立了准确的保留时间预测模型 (ΔtR <0.33分钟,MRE ∼3.4%).
  • 能够覆盖190个子类中的21,700多种脂质,具有高灵敏度,精度,准确性和稳定性.
  • 在各种生物基质 (人体血,尿液,癌细胞,细菌,植物,肝脏组织,便) 中证明适用性.

结论:

  • 开发的UHPLC-MS/MS方法提供了高覆盖率的定量脂组学.
  • 这种方法克服了脂质分析的先前局限性,特别是酸性脂质.
  • 为研究健康和疾病中的脂质功能提供了强大的工具.