在生物系统中通过基于纳夫他胺的光探针快速视觉检测histidine
Chen Xu1, Fang Zeng1, Shuizhu Wu1
1State Key Laboratory of Luminescent Materials and Devices, Guangdong Provincial Key Laboratory of Luminescence from Molecular Aggregates, College of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, China.
概括
一个新的光探针快速和视觉检测histidine (His),一个必不可少的氨基酸. 这种快速,有选择性的传感器在生物样本中工作,并使现场诊断和营养监测成为可能.
科学领域:
- 分析化学 分析化学
- 生物化学 生物化学
- 分子传感传感器是一种分子传感器.
背景情况:
- 伊斯蒂丁 (His) 是一种必需的氨基酸,对代谢途径至关重要.
- 它作为代谢障碍,营养状况和疾病进展的生物标志物.
- 对于临床和农业应用,需要准确和快速的His检测方法.
研究的目的:
- 开发一种新的光探针,用于快速,选择性和视觉检测histidine.
- 为了证明探测器在各种生物矩阵中的实用性以及现场传感.
主要方法:
- 合成了一种基于纳夫他胺-二聚胺的光探针 (DPA-BID-Cu2+).
- 探测器使用Cu2+复合物被histidine破坏,导致"启动"的光反应.
- 使用UV-Vis光谱和光显微镜进行检测.
主要成果:
- 探测器在20秒内实现了实时胺检测.
- 它表现出很大的斯托克斯转移 (110纳米) 清晰的信号检测.
- 探测器在各种生物样本 (尿液,活细胞,植物组织) 中显示出高选择性和灵敏性,无需预处理.
- 一个便携式双管装置被开发用于现场检测.
结论:
- 开发的光探测器提供了一个高效,快速响应和现场部署的平台,用于histidine传感.
- 这项技术对临床诊断,营养监测和现场生物分析具有重大前景.
- 探测器有助于直接可视化和现场分析,将分子传感与实际应用相结合.
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