氨酸诱导的细胞外聚合物物质破坏驱动了代谢重编程,并在无氧污泥发酵中增强了挥发性脂肪酸的产生
Jingyang Luo1, Shumeng Yang1, Qian Feng1
1Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China; College of Environment, Hohai University, Nanjing 210098, China.
Bioresource technology
|March 3, 2026
概括
通过破坏蛋白质和重编程微生物群落,抗微生物三素意外地促进了废物活性污泥中的挥发性脂肪酸 (VFA) 生产. 这通过代谢适应和增加基质可用性来增强资源回收.
科学领域:
- 环境微生物学 环境微生物学
- 生物技术是生物技术.
- 生物地质化学生物地质化学
背景情况:
- 废物活性污泥 (WAS) 中的抗菌污染物挑战无氧发酵.
- 连接抗微生物污染物与挥发性脂肪酸 (VFA) 生产的机制尚不清楚.
研究的目的:
- 调查1,3,5-triazine (triazine) 在WAS无氧发酵过程中对VFAs产生的影响.
- 阐明三素诱导的代谢和微生物转变的潜在机制.
主要方法:
- 度依赖的实验与三素暴露.
- 对VFA,细胞外聚合物 (EPS) 和的分析.
- 蛋白质-氨酸相互作用的分子对接.
- 高通量16S rRNA和功能元基因组 (PICRUSt2) 分析.
- 部分最小平方路径建模.
主要成果:
- 矛盾的是,triazine增加了VFAs的产量,达到1771毫克的COD/L (18.4倍的增加).
- 由蛋白质水解和EPS破坏驱动的代谢转向乙酸主导 (54.3%).
- 微生物群落的重组,富含蛋白质和酸盐生产细菌,并抑制甲基生物.
- 调节蛋白质和乙-CoA合成基因的升级,并激活定数感应系统.
结论:
- 通过EPS介导的代谢重编程,来自triazine的抗微生物压力可以增强WAS中的资源回收.
- 由微生物适应驱动的基质可用性是三酸压力下VFAs积累的主要因素.
- 氨酸通过破坏微生物社区的结构和功能来促进VFA的产生,有利于乙酸盐生物合成.
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