相关实验视频

Updated: May 10, 2026

Using X-ray Crystallography, Biophysics, and Functional Assays to Determine the Mechanisms Governing T-cell Receptor Recognition of Cancer Antigens

Using X-ray Crystallography, Biophysics, and Functional Assays to Determine the Mechanisms Governing T-cell Receptor Recognition of Cancer Antigens

Published on: February 6, 2017

癌症免疫学和免疫治疗中的TCR测序:什么,什么时候,在哪里,为什么,以及如何

Yohei Nose^1,2, Igor Figueiredo^1,2, Kevin Tuballes^1,2

¹Immunology and Immunotherapy, Icahn School of Medicine at Mount Sinai, New York, New York, USA.

Journal for immunotherapy of cancer

|March 3, 2026

概括

T细胞受体测序 (TCR-seq) 通过追踪T细胞克隆的时间和空间来分析适应性免疫力. 这种强大的工具提供了对免疫反应,疾病进展和治疗疗效的见解,尽管持续需要改进抗原特异性预测.

科学领域:

免疫学免疫学免疫学
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

T细胞受体 (TCR) 对适应性免疫至关重要,由V(D) J重组产生,为免疫监测创造必要的多样化谱.
TCR测序 (TCR-seq) 是表征自适应性免疫谱系的关键技术,为T细胞多样性,抗原特异性和克隆动态提供了洞察力.

研究的目的:

审查目前的TCR测序技术和应用的现状.
突出最近的技术进步和免疫谱系分析中的新兴应用.
通过什么,什么时候,在哪里,为什么,以及如何使用的镜头来检查TCR-seq.

主要方法:

审查各种TCR-seq平台,包括基于DNA和基于RNA的协议,短读和长读测序,以及批量与单细胞方法.
讨论计算方法,如机器学习,从TCR-seq数据推断抗原特异性.
分析TCR-seq的时间和空间跟踪能力,用于监测免疫反应和T细胞贩运.

主要成果:

TCR-seq使T细胞克隆动态的纵向监测和不同解剖部位的免疫列表的空间分析成为可能.
空间TCR-seq的进步将空间上下文与克隆身份相结合,揭示了组织特异性免疫反应.
目前用于预测抗原特异性的方法需要进一步提高准确性和通用性.

结论:

TCR-seq是理解适应性免疫的多功能工具,为T细胞行为和健康和疾病中的免疫反应提供了深入的见解.
技术进步继续提高TCR-seq.的分辨率,吞吐量和分析能力.
未来的研究应该专注于改善抗原特异性预测,并整合各种TCR-seq方法进行全面的免疫谱系分析.

关键词:

生物标志物生物标志物免疫检查点抑制剂免疫检查点抑制剂下一代测序 - - 国家基因系统 (NGS)一个T细胞细胞.T细胞受体 - - TCR 细胞受体

更多相关视频

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

Dissociation of Human and Mouse Tumor Tissue Samples for Single-cell RNA Sequencing

Dissociation of Human and Mouse Tumor Tissue Samples for Single-cell RNA Sequencing

Published on: August 16, 2024

相关概念视频

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

Cancer Vaccines

Cancer Vaccines

Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...