基底前脑胆协神经元的活动依赖调节由条状脊柱投射神经元进行
Linqing Sun1,2, Enrico Zampese1, Tristano Pancani1
1Department of Neuroscience, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA.
概括
状脊柱投射神经元 (SPNs) 调节基底前脑胆协神经元 (BFCNs). D1R-SPNs提供了显著的控制,持续的刺激通过塔基宁和酸感应离子通道导致激发,影响认知和兴奋.
科学领域:
- 神经科学是一个神经科学.
- 细胞和分子神经科学
- 系统神经科学 系统神经科学
背景情况:
- 基本前脑胆能神经元 (BFCN) 对认知,注意力和睡眠-清醒周期至关重要.
- 控制BFCN活动的特定神经回路仍然不完全理解.
- 已知状脊状投射神经元 (SPN) 投射到基底前脑.
研究的目的:
- 描述控制基底前脑胆能神经元 (BFCN) 尖端的 afferent 连接.
- 研究不同脊状投射神经元 (SPN) 亚型在调节BFCN中的功能性作用.
- 阐明调解SPN引起的BFCN响应的下游机制.
主要方法:
- 单突触狂犬病病毒追踪将SPN输入映射到BFCN.
- 在ex vivo脑切片中对D1R-SPN和D2R-SPN轴突进行光遗传刺激.
- 电生理学记录以评估BFCN对SPN刺激的反应.
- 药理学操作以确定受体参与 (NK1Rs,ASICs).
主要成果:
- D1多巴胺受体表达SPNs (D1R-SPNs) 和D2多巴胺受体表达SPNs (D2R-SPNs) 均激活BFCNs.
- 与D2R-SPN相比,D1R-SPN的光遗传激活在BFCN中引起了更强烈的反应.
- 暂时的D1R-SPN刺激导致GABAergic抑制,而持续的刺激导致通过NK1Rs和ASICs激发.
结论:
- 一个分布式的D1R-SPN群体显著调节BFCN活动.
- 通过SPN介导的BFCN调节涉及复杂的抑制和刺激途径.
- 这些发现凸显了SPNs在调节兴奋,认知和睡眠方面的新角色.
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