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在风湿性关节炎中,METTL3-Mediated m6A 循环调节TS4/miR-146b-3p轴

Shu Li¹, MengYu Zhang¹, SiYu Liang¹

¹The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, China.

|March 3, 2026

概括

这项研究表明,METTL3介导的N6-甲基氨酸 (m6A) 修饰调节了类风湿性关节炎 (RA) 中的circINTS4/miR-146b-3p轴. CircINTS4作为RA严重程度的生物标志物,提供潜在的治疗点.

科学领域:

表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
非编码RNA生物学
类风湿病学类风湿病学

背景情况:

类风湿性关节炎 (RA) 涉及突炎和关节破坏.
在RA病变发生过程中表观遗传调节和非编码RNA网络尚未完全理解.
这项研究探讨了RA中的N6-甲基氨酸 (m6A) 修饰和circINTS4的研究.

研究的目的:

研究m6A修饰和circINTS4在类风湿性关节炎中的作用.
在RA病变发生过程中阐明circINTS4/miR-146b-3p轴.
确定RA的潜在诊断和治疗点.

主要方法:

在RA中对m6A和miR-146家族研究的图书统计分析.
全转录组测序以识别RA突组织中差异表达的circRNAs.
功能丧失实验和双化酶报告员试验,以检查METTL3-circINTS4-miR-146b-3p相互作用.
在RA纤维细胞样同胞细胞 (RA-FLS) 和体内模型中进行功能测试,以评估circINTS4对RA进展的影响.

主要成果:

在RA患者中,circINTS4显著下调,而miR-146b-3p显著上调.
METTL3 调节 circINTS4 的 m6A 修改,影响 circINTS4 和 miR-146b-3p 的水平.
circINTS4作为miR-146b-3p的分子海绵,影响RA-FLS的扩散,迁移和亡.
在生物体内,circINTS4的枯竭加剧了结膜炎症和促炎性细胞因子水平.

结论:

通过METTL3介导的m6A修饰调节了RA中的circINTS4/miR-146b-3p轴.
circINTS4调节了RA-FLS的致病性和炎症反应.
circINTS4是一种潜在的RA疾病严重程度和治疗点的生物标志物.

关键词:

循环RNA (循环RNA) 是一种循环RNA.在METTL3中,METTL3是METTL3的第一个类型.在 MiR-146b-3p 里面,我们可以看到 MiR-146b-3p.N6-甲基氨酸 (m6A) 的修改类风湿性关节炎 (RA) 是一种

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