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人工激活临床失败的人类卵细胞可以使欧卵母细胞形成

Marcos Iuri Roos Kulmann¹, Gerta Frantz², Norma Pagnoncelli de Oliveira²

¹Nilo Frantz Reproductive Medicine, Porto Alegre, Brazil. marcos@nilofrantz.com.br.

Journal of assisted reproduction and genetics

|March 3, 2026

概括

在临床上失败的人类卵细胞可以重新使用,以创建用于干细胞研究的欧类胚胎细胞. 这种伦理来源的胚胎资源尽管在获得干细胞系方面存在挑战,但仍有希望.

科学领域:

生殖生物学和干细胞研究.

背景情况:

临床失败的人类卵细胞通常在细胞内质精子注射 (ICSI) 后被丢弃.
产生在伦理上不受限制的人类胚胎干细胞 (hESC) 和亲遗传胚胎干细胞 (phESC) 资源对研究至关重要.

研究的目的:

通过体外成熟 (IVM) 和人工卵细胞激活 (AOA) 来研究利用临床失败的卵子细胞用于胚胎细胞生成的潜力.
评估这些胚胎囊对于hESC和phESC衍生的适用性.

主要方法:

一项前性研究涉及716个未成熟或未能受精的卵子,来自185名接受ICSI的患者.
卵细胞接受了IVM,然后使用离子和6-DMAP进行AOA.
对胚胎发育进行了监测,对胚胎囊进行了 ploidy (PGT-A) 的评估,并且在无料条件下培养了内部细胞群.

主要成果:

人工卵细胞激活 (AOA) 导致65.9%的激活率和4.8%的芽细胞形成.
与IVM卵子细胞相比,未能受精 (FF) 卵子细胞显示出更高的效率 (77.9%激活,7.2%芽细胞).
在15个形成的芽细胞囊中,近一半 (47%) 是欧体,证实了生命力;然而,没有成功衍生出任何干细胞系.

结论:

临床失败的卵子细胞的人工卵细胞激活可以产生潜在适合hESC和phESC衍生的euploid母细胞囊.
在无料条件下衍生干细胞系仍然具有挑战性.
这一策略为扩大干细胞资源提供了一种道德合理和可行的方法,特别是在胚胎研究受到法律限制的地方.

关键词:

人工卵子细胞激活欧普洛伊德胚胎囊是如何形成的没有受精的卵子细胞.人类胚胎干细胞的人类胚胎干细胞在体外成熟.

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