相关实验视频

Updated: May 7, 2026

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques

Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4⁺ T Cells Isolated from Rhesus macaques

Published on: September 25, 2018

HIV-seq揭示了艾滋病毒病毒和被抑制的艾滋病毒感染者的HIV转录细胞之间的基因表达差异

Julie Frouard^1,2, Sushama Telwatte^3,4, Xiaoyu Luo^1,2

¹Gladstone Institutes, San Francisco, CA, USA.

Nature communications

|March 3, 2026

概括

一种名为HIV-seq的新方法增强了在艾滋病毒感染者 (PWH) 中检测HIV转录细胞的能力. 这项技术揭示了这些细胞如何在抗逆转录病毒疗法 (ART) 期间持续存在并导致炎症.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
分子生物学分子生物学

背景情况:

艾滋病毒转录细胞有助于慢性炎症和艾滋病毒感染者 (PWH) 的病毒反弹.
研究这些细胞是具有挑战性的,因为它们的低频率和低水平的非多聚氨基化HIV转录.
目前的方法,如单细胞RNA测序 (scRNA-seq),在检测这些细胞方面存在局限性.

研究的目的:

开发和验证一种新的方法,HIV-seq,用于更好地检测HIV转录细胞.
在病毒性和抗逆转录病毒疗法 (ART) 抑制期间描述HIV转录细胞的细胞和分子特征.
了解艾滋病毒在这些细胞中的持久性机制.

主要方法:

开发HIV-seq,一种修改后的scRNA-seq技术,涉及入捕获序列,针对保存的HIV区域.
在病毒病和ART抑制期间,对来自HIV感染者 (PWH) 的样本进行HIV-seq的应用.
对HIVRNA+细胞频率,T细胞子集和转录特征 (细胞因子信号) 的分析.

主要成果:

与标准的scRNA-seq.相比,HIV-seq每细胞HIV读数的平均数量翻了一番.
在病毒病和ART抑制期间,HIV RNA+细胞在T效应记忆细胞中得到丰富.
在病毒病期间,HIV RNA+细胞表现出细胞毒性特征,而在ART抑制期间,它们表现出抗炎特征,TGF-β升高,IFN信号减少.

结论:

HIV-seq是一种有效的工具,可以增强对HIV转录细胞的研究.
在病毒病和ART抑制期间,HIV转录细胞表现出不同的功能状态.
了解这些细胞状态对于开发管理艾滋病毒持久性和炎症的策略至关重要.

更多相关视频

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Published on: October 16, 2018

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Exploring m⁶A and m⁵C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

相关概念视频

Ribosome Profiling

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

Size and Structure of Viral Genomes

Size and Structure of Viral Genomes

Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...

Viruses with RNA Genomes

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...

Human Virome

Human Virome

The human body harbors a vast and diverse viral community known as the human virome. The virome includes bacteriophages that infect bacteria, and eukaryotic viruses that infect human cells. Transient dietary and environmental viruses also contribute to this dynamic ecosystem. Estimates suggest the human body may contain on the order of 10¹³ viral particles, though abundance varies widely by body site and detection method.Comprehensive characterization of the virome has become possible only with...