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在油性酵母Rhodosporidium toruloides中使用IPP绕道途径评估异二醇的产量

Valentina E Garcia^1,2, Gina M Geiselman^1,2, Hee Jin Hwang^1,2

¹Joint BioEnergy Institute, Emeryville, CA, 94608, USA.

Biotechnology for biofuels and bioproducts

|March 3, 2026

概括

这项研究探讨了用于先进航空燃料的Rhodosporidium toruloides酵母中的异二醇生产. 优化的菌株在实验室介质和水解液中都取得了显著的异二醇产量.

科学领域:

生物技术是生物技术.
合成生物学合成生物学
代谢工程是代谢工程.

背景情况:

国内航空燃料的需求不断增长,需要先进的生物衍生替代品.
异二醇是新型航空燃料的关键前体,其先前生产已在模型生物中证明.
需要进一步探索工业相关酵母菌株中异二醇生物生产的可能性.

研究的目的:

为了评估和优化酵母Rhodosporidium toruloides中的异二醇产量.
调查梅瓦诺酸路径流量和IPP绕过路径工程对异二醇标位的影响.
识别和解决R. toruloides.的潜在生产瓶.

主要方法:

在R. toruloides.中使用IPP旁路的工程化异二醇生产.
代谢流量分析和蛋白质组学以确定生产限制.
通过探索IPP-绕道途径的替代酶来优化菌株.
使用胆 lysinate 在水解盐中发酵.

主要成果:

在R. toruloides中使用已建立的IPP-绕道途径取得了成功的异二醇生产.
增加的美瓦酸路径流量只导致异二烯醇标位的适度改善.
蛋白质组分析在IPP-绕道的最后一步发现了一个瓶,这导致了对替代酶的探索.
产量最高的R. toruloides菌株在模拟介质中产生了93.1 mg/L的异二醇,在水解液中产生了27.3 mg/L.

结论:

确定了Rhodosporidium toruloides中异二醇生产的可行性.
确定了进一步代谢工程的关键领域,以提高异二醇产量.
从农业原料中生物生产异二醇的未来发展奠定了基础.

关键词:

先进的航空燃料先进的航空燃料.通过IPP绕过路径.异二醇是一种异二醇.酸酶是一种酸酶.罗多斯杆菌 (Rhodosporidium toruloides) 是一种多类植物.

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