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在临床便样本中对Midi Parasep®无溶剂和Ritchie度技术进行比较评估,用于虫和原生动物的可视化

Carles Rubio Maturana¹, Francesc Zarzuela Serrat¹, Lidia Goterris²

¹Microbiology Department, Vall d'Hebron University Hospital, Vall d'Hebron Research Institute (VHIR), Barcelona, Spain.

Parasites & vectors

|March 3, 2026

概括

里奇方法在检测虫方面比Midi Parasep®无溶剂 (SF) 系统更敏感,尽管这两种方法都能准确检测原生动物. 便度技术的选择取决于临床背景和可疑的寄生虫负担.

科学领域:

临床微生物学临床微生物学
寄生虫学的寄生虫学
诊断性绩效评估的诊断性绩效评估是如何进行的

背景情况:

便检查对于诊断寄生虫感染至关重要.
精确检测虫和原生动物依赖于有效的度技术.
对比诊断方法对于优化实验室工作流程至关重要.

研究的目的:

为了比较Midi Parasep®无溶剂 (SF) 系统和Ritchie方法用于便样本分析的诊断性能.
根据寄生虫负担和流行病学来评估不同便度技术的适用性.
在各种临床实验室环境中为选择最佳诊断方法提供信息.

主要方法:

一项回顾性研究分析了100个虫阳性便样本.
样品最初使用Ritchie技术进行识别,并使用Midi Parasep® SF系统进行再处理.
使用统计分析 (Z测试或费舍尔精确测试) 来比较结果.

主要成果:

里奇方法检测出更多的寄生虫病因 (139) 比Midi Parasep® SF系统 (85),总体一致性为61.15%.
原生动物检测显示出100%的一致性,但虫检测一致性明显较低 (54.6%).
Midi Parasep® SF对虫,特别是较大的虫和低寄生虫负担样本的敏感性降低. 固剂AlcorfixTM引起了一些形态变化.

结论:

里奇方法对虫检测更为敏感,特别是在移民或旅行医学中的疑似病例中.
Midi Parasep® SF对原生动物有效,适用于高通量设置.
临床背景和疑似寄生虫感染应指导便度技术的选择;补充方法如血清学可能有助于诊断.

关键词:

临床便样本临床便样本这就是Midi Parasep®.光学显微镜是一种光学显微镜.原生动物原生动物里奇的方法是里奇的方法.在土壤中传播的虫.

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