硫化纳米级零价值铁激活皮质酸盐对细菌失活和结合基因转移的影响
Li Liang1,2, Weiying Li2
1Key Laboratory of Water Security and Water Environment Protection in Plateau Intersection, Ministry of Education, Key Laboratory of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Northwest Normal University Lanzhou 730070 PR China.
RSC advances
|March 4, 2026
概括
硫化纳米级零价值铁 (S-nZVI) 激活的周期酸盐有效地禁用了耐抗生素的细菌,并减少了基因转移. 这种S-nZVI/PI系统提供了一种新的方法来打击环境中的抗生素耐药性.
科学领域:
- 环境科学与工程环境科学与工程
- 微生物学 微生物学
- 材料科学 材料科学 材料科学
背景情况:
- 由于过度使用抗生素,抗生素耐药性 (AR) 构成了严重的全球健康威胁.
- 抗生素耐药细菌 (ARB) 和它们的移动遗传元件,抗生素耐药基因 (ARG),有助于耐药性的传播.
- 需要有效的补救策略来控制污染环境中的ARB和ARG.
研究的目的:
- 评估硫化纳米级零价值铁 (S-nZVI) 激活周期酸盐 (PI) 在非活性化ARB的疗效.
- 评估S-nZVI/PI系统对ARG结合性转移的影响.
- 阐明S-nZVI/PI抑制ARG结合转移的机制.
主要方法:
- 实验是使用E.进行的. 大肠杆菌菌株HB101 (捐赠者) 和NK5449 (接受者).
- 在特定度 (S-nZVI = 50 mg L-1 ,PI = 1.0 mM) 应用S-nZVI/PI系统40分钟.
- 细菌无活化,ARG结合转移频率和关键转移基因的表达量化.
主要成果:
- 该S-nZVI/PI系统显著降低了ARG结合转移频率从6.3×10−4到6.6×10−5.
- 抑制腺三酸盐 (ATP) 合成和关键基因表达 (traF,trbA,traG,traJ,korA,korB) 被确定为机制.
- 较高的S-nZVI和PI剂量增加了细菌无活化,但对ARG转移的影响较小;较低的初始细菌度提高了效率.
结论:
- 该S-nZVI/PI系统是一个有前途的技术,用于禁用ARB和抑制ARG结合转移.
- 这种补救方法提供了一种可行的策略,用于控制受污染的生态系统中抗生素耐药性的传播.
- 进一步的研究可以优化S-nZVI/PI应用,用于ARB和ARG的环境修复.
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