番茄激活乙烯信号,以维持与致病相关的基因的表达,以赋予细菌的枯耐药性
Weiwei Cai1, Qiquan Li1, Feina Liu1
1Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang Agriculture and Forestry University, Hangzhou, Zhejiang, China.
耐细菌枯的番茄品种可以激活与病变发生相关的 (PR) 蛋白质和转录因子. 乙烯合成是对抗Ralstonia solanacearum的持续防御的关键,有助于番茄耐药性繁殖.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 由Ralstonia solanacearum*引起的细菌,是番茄生产的重大威胁.
- 抗病品种提供了一种有希望的策略来控制番茄中的细菌枯.
- 了解阻力背后的分子机制对于培育改进的番茄品种至关重要.
研究的目的:
- 在R. solanacearum*感染后,研究耐药和易受性番茄品种的基因表达特征.
- 为了确定关键的基因和途径,涉及到番茄对细菌枯的耐药性.
- 阐明乙烯生物合成在植物防御反应中的作用.
主要方法:
- 从耐药 ('ZJ-7', '04056') 和易受 (Ailsa Craig cv. 从耐药 ('ZJ-7', '04056') 和易受 (Ailsa Craig cv. - AC) 在注射后3个小时和6个小时 (hpi) 与R. solanacearum*.
- 对基因表达水平的分析,重点关注与病变发生相关的 (PR) 蛋白质和转录因子.
- 评估以太 (乙烯释放化合物) 应用对敏感品种AC的基因表达的影响.
主要成果:
- 与敏感AC相比,耐药品种"ZJ-7"和"04056"显示PR基因和转录因子的表达显著增加.
- 敏感的AC表现出对R. solanacearum*感染的延迟和较少的转录反应.
- 两种耐药品种都显示了与乙烯生物合成相关的基因的激活;在AC中应用ethephon模仿了一些耐药反应.
结论:
- 番茄对细菌枯的持续防御与乙烯合成密切相关.
- 乙烯信号通路在耐药番茄品种对R. solanacearum的反应中起着至关重要的作用.
- 这些发现为通过针对乙烯生物合成的育种策略增强番茄对细菌枯的耐药性提供了理论基础.
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