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血清中的乙烯基醇测定:对真实样本上的酶定量和GC-MS进行比较

Marine Deville¹, Corinne Charlier¹

¹Laboratory of Clinical, Forensic and Environmental Toxicology, Center for Interdisciplinary Research on Medicines (CIRM), University Hospital of Liege, Liege, Belgium.

Clinical toxicology (Philadelphia, Pa.)

|March 4, 2026

概括

一种新的乙烯糖醇酶测定方法可以在血清中快速检测,从而改善患者的护理. 虽然高度敏感和特异性,积极的结果需要通过染色学确认.

科学领域:

临床化学临床化学
毒理学毒理学毒理学
分析化学分析化学

背景情况:

乙烯糖醇中毒是危及生命的,具有不可靠的临床症状.
诊断通常依赖于血清检测,但黄金标准色谱很难用于常规使用.

研究的目的:

在自动化分析仪上实施和评估乙烯糖醇的酶定量.
通过使用真实患者样本,在10年的时间内比较酶定量测试与气色谱-质谱的性能.

主要方法:

开发了一种测量乙烯基甘醇氧化成甘醇的酶分析法.
酶定量测试的结果通过气色谱-质谱证实了.
这两种方法都具有50 mg/L的下限量化值.

主要成果:

在405个独特的患者样本中,34个通过这两种方法检测出阳性 (8.4%真阳性).
与染色体相比,酶测试显示了89%的灵敏度和83%的特异性.
假阳性 (15.3%) 往往低于治疗值,其中一些是由乳酸或LDH水平解释的.

结论:

酶检测可快速检测乙烯糖醇,从而能够及时做出临床决定.
观察到高灵敏度和特异性,尽管建议通过染色体检查进行确认以获得积极结果.
缩短的周转时间是酶分析在临床实践中的重要优势.

关键词:

自动化分析分析自动化分析酶性检测法酶性检测法乙烯基甘醇是一种乙烯基甘醇.气相色谱是一种气相色谱.酒精中毒,中毒的情况.

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