在出生后发育期间,人类和小鼠之间的心血管缩放
Raymond J Martin1, Bruno V Rego1
1Cardiovascular Engineering Lab, Department of Biological & Agricultural Engineering, Louisiana State University, Baton Rouge, LA, USA.
概括
将小鼠研究结果转化为人类是具有挑战性的,因为不同的发展时间表. 这项研究得出了小鼠心血管发育的年龄缩放因素,提高了研究相关性.
科学领域:
- 心血管生物学 心血管生物学
- 发展生物学 发展生物学
- 翻译医学是一种翻译医学.
背景情况:
- 人类和小鼠模型之间的准确年龄匹配对于翻译研究结果至关重要,特别是在发育研究中.
- 各种物种发育时间表中的特定组织变异使年龄等价性复杂化,特别是在成熟之前.
研究的目的:
- 开发人类和小鼠之间的心血管特异性年龄等效映射.
- 建立一个框架,在正常心血管发育和儿科心血管疾病的研究中选择适当的老鼠年龄.
主要方法:
- 从产后发育研究中编译和数学建模心血管解剖,功能和生物力学数据.
- 确定最佳的年龄尺度因子,以调整人类和小鼠的发育时间课程,以满足各种心血管变量.
主要成果:
- 确定了每年0.69个小鼠周的人类年龄的总体最佳年龄因子,个人最佳情况从0.4到1.3 wk/yr.
- 没有一个单一的通用缩放因子可以完全调整所有发展时间表,因为物种特定的变化.
- 一些心血管变量,如心率和大动脉壁剪切应力,显示质量上不同的时间过程,不能通过缩放来调整.
结论:
- 对于专注于特定组织和负载条件的研究,建议使用变量特定的年龄缩放因子.
- 识别的年龄尺度因子增强了从心血管发育小鼠模型中发现的结果的翻译相关性和解释.
- 存在局限性,因为并非所有发育轨迹都能完美一致,因此需要仔细考虑所选择的缩放因子.
相关概念视频
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01:07Development of Blood Vessels
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