内皮细胞分化相关的circRNAs驱动血管内皮细胞的分化
Yu Sun1, Zhuona Yin2, Yidan Huang1
1Department of Cardiac Intensive Care Unit, the Cardiovascular Hospital, the Affiliated Guangdong Second Provincial General Hospital of Jinan University, No. 466, Xingang Middle Road, Haizhu District, Guangzhou, 510310, Guangdong, China.
Biochemical genetics
|March 4, 2026
概括
循环RNAs (circRNAs) 调节血管内皮细胞的分化. Hsa_circ_0090122通过与特定的微RNA相互作用来调节ADAMTS9表达来促进内皮细胞分化.
科学领域:
- 分子生物学分子生物学
- 心血管研究研究心血管研究
- 干细胞生物学 干细胞生物学
背景情况:
- 内皮功能障碍与心血管和脑血管疾病有关.
- 控制血管内皮细胞分化的精确分子机制尚不清楚.
- 循环RNAs (circRNAs) 正在成为细胞过程中的关键调节者.
研究的目的:
- 研究血管内皮细胞分化中的circRNAs的功能和调节机制.
- 确定特定的circRNA及其相关途径,参与干细胞分化成内皮细胞.
- 阐明竞争的内源性RNA (ceRNA) 网络调节内皮细胞分化.
主要方法:
- 在诱导多能干细胞 (iPSC) 分化成内皮细胞过程中对差异表达的circRNAs (DECs) 和基因 (DEGs) 的查.
- 对DECs的微RNA (miRNA) 目标的生物信息预测.
- 在细胞和有机体层面验证circRNA和基因表达.
- 对涉及circRNAs,miRNAs和信使RNAs (mRNAs) 的ceRNA调节轴的分析.
主要成果:
- 鉴定了六个核心基因,并选择了ADAMTS9 (一种具有血栓胺基因的分解蛋白和金属蛋白酶9) 进行进一步研究.
- 在内皮细胞分化过程中,Hsa_circ_0090122和ADAMTS9的mRNA/蛋白质水平增加.
- Hsa_miR-548az-5p和hsa-miR-548t-5p水平下降,这与hsa_circ_0090122和ADAMTS9.9的ceRNA相互作用一致.
- Hsa_circ_0090122表现出显著的表达变化,与ADAMTS9和特定的miRNA相关.
结论:
- Hsa_circ_0090122在促进血管内皮细胞分化方面发挥着至关重要的作用.
- hsa_circ_0090122/hsa-miR-548az-5p/hsa-miR-548t-5p/ADAMTS9轴是内皮细胞分化的关键调节途径.
- 了解这种circRNA介导的ceRNA网络为心血管疾病提供了潜在的治疗点.
相关概念视频
Regulation of Angiogenesis and Blood Supply
3.8K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.8K
Mechanism of Angiogenesis
7.3K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
7.3K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal
2.8K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.8K
Overview of the Vascular System
3.7K
The vascular system comprises an extensive network of arteries, capillaries, and veins. The vascular system can be broadly divided into the blood and lymphatic systems. Typically, blood vessels can be categorized into three histological regions: tunica intima, tunica media, and tunica adventitia. The tunica intima consists of a single layer of endothelial cells attached to the basal lamina. Underlying the basal lamina is a connective tissue layer and an elastic lamina that gives stability and...
3.7K
Cellular Differentiation
6.0K
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...
A zygote is a...
6.0K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.5K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.5K


